Single cell gel electrophoresis assay: methodology and applications

被引：410

作者：

Rojas, E ^{[1
]}

Lopez, MC ^{[1
]}

Valverde, M ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Genet & Toxicol Ambiental, Lab Genet & Toxicol Mol, Mexico City 04510, DF, Mexico

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1999年 / 722卷 / 1-2期

关键词：

reviews; single cell gel electrophoresis; Comet assay; DNA;

D O I：

10.1016/S0378-4347(98)00313-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method for DNA double- and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukariotic individual cells. Given its overall characteristics, this method has been widely used over the past few years in several different areas. In this paper we review the studies published to date about the principles, the basic methodology with currently used variations. We also explore the applications of this assay in: genotoxicology, clinical area, DNA repair studies, environmental biomonitoring and human monitoring. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：225 / 254

页数：30

共 292 条

[1] Photochemical and photobiological studies of a furonaphthopyranone as a benzo-spaced psoralen analog in cell-tree and cellular DNA [J].