Determination of γ-globulin at nanogram levels by its enhancement effect on the resonance light scattering of functionalized HgS nanoparticles

被引：29

作者：

Wang, LY ^{[1
]}

Wang, L ^{[1
]}

Dong, L ^{[1
]}

Hu, YL ^{[1
]}

Xia, TT ^{[1
]}

Chen, HQ ^{[1
]}

Li, L ^{[1
]}

Zhu, CQ ^{[1
]}

机构：

[1] Anhui Normal Univ, Coll Chem & Mat Sci, Wuhu 24100, Peoples R China

来源：

TALANTA | 2004年 / 62卷 / 02期

关键词：

resonance light scattering; gamma-globulin; nano-HgS;

D O I：

10.1016/j.talanta.2003.07.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel assay of gamma-globulin (gamma-IgG) with a sensitivity at the nanogram level is proposed based on the measurement of enhanced resonance light-scattering (RLS) signals resulting from the interaction of functionalized nano-HgS with gamma-globulin. At pH 5.03, the RLS signals of functionalized nano-HgS were greatly enhanced by gamma-globulin in the region of 200-700 nm characterized by the peak around 362 nm. Linear relationship can be established between the enhanced RLS intensity and gamma-globulin concentration in the range of 10-140 ng ml(-1). The limit of detection is 2.71 ng ml(-1). Based on this, a new direct quantitative determination method for gamma-globulin in blood serum samples without separation of human serum albumin (HSA) is established. The contents of gamma-IgG in blood serum samples were determined with recovery of 95.7-102.5% and R.S.D. of 1.6-2.4%. This method is proved to be very sensitive, rapid, simple and tolerance of most interfering substances. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：237 / 240

页数：4

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