Transcriptional analysis of the PTEN/MMAC1 pseudogene, ΨPTEN

被引：73

作者：

Fujii, GH ^{[1
]}

Morimoto, AM ^{[1
]}

Berson, AE ^{[1
]}

Bolen, JB ^{[1
]}

机构：

[1] DNAX Res Inst Mol & Cellular Biol Inc, Dept Cellular Signaling, Palo Alto, CA 94304 USA

来源：

ONCOGENE | 1999年 / 18卷 / 09期

关键词：

MMAC; PTEN; tumor suppressor; pseudogene;

D O I：

10.1038/sj.onc.1202492

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PTEN/MMAC1 is a recently characterized tumor suppressor. A pseudogene derived from the human PTEN/MMAC1 phosphatase, Psi PTEN, has been reported. Recent analysis of the pseudogene revealed conflicting results about the expression of Psi PTEN. In this study, we show that the PTEN/MMAC1 pseudogene is actively transcribed in all cells and tissues examined. In some cases, pseudogene transcripts were found to represent as much as 70% of the total PTEN/MMAC1 RNA. As Psi PTEN is transcribed, there is a potential for misinterpretation of PTEN/MMAC1 mutations when RT - PCR techniques are used, as well as potential for a Psi PTEN-encoded translation product, Although we were unable to detect a pseudogene protein product in the cell lines examined, a baculovirus produced GST pseudogene fusion protein exhibited phosphatase activity comparable to wild type. The results of this study, taken together, indicate the potential complication of PTEN/MMAC1 molecular analysis caused by the expression of Psi PTEN.

引用

页码：1765 / 1769

页数：5

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