Genistein inhibits angiogenesis developed during rheumatoid arthritis through the IL-6/JAK2/STAT3/VEGF signalling pathway

被引：69

作者：

Cheng, Wen-Xiang ^{[1
,2
,13
]}

Huang, Huan ^{[1
,2
,11
]}

Chen, Jian-Hai ^{[1
,2
]}

Zhang, Tian-Tian ^{[8
]}

Zhu, Guo-Yuan ^{[4
]}

Zheng, Zheng-Tan ^{[1
]}

Lin, Jie-Tao ^{[1
,2
]}

Hu, Yi-Ping ^{[1
]}

Zhang, Yong ^{[7
]}

Bai, Xue-Ling ^{[1
]}

Wang, Yan ^{[1
]}

Xu, Zhan-Wang ^{[9
]}

Song, Bing ^{[1
,10
]}

Mao, Yi-Ying ^{[12
]}

Yang, Fei ^{[6
]}

Zhang, Peng ^{[1
,2
,3
,5
,13
]}

机构：

[1] Chinese Acad Sci, Shen Zhen Inst Adv Technol, Ctr Translat Med Res & Dev, 1068 Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 10049, Peoples R China

[3] Univ Chinese Acad Sci, Shenzhen Hosp, Shenzhen 518000, Peoples R China

[4] Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Macau, Peoples R China

[5] Univ Chinese Acad Sci, Shenzhen Hosp, Dept Orthoped, Shenzhen 518000, Peoples R China

[6] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Key Lab Orthopaed Implant,Dept Orthopaed, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China

[7] Shenzhen Pingle Orthopaed Hosp, Shenzhen 518000, Guangdong, Peoples R China

[8] Peoples Hosp Baoan Dist, Dept Rheumatol, Shenzhen 518128, Guangdong, Peoples R China

[9] Shandong Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Orthopaed, Jinan 250000, Shandong, Peoples R China

[10] Cardiff Univ, Sch Dent, Heath Pk, Cardiff CF23 6AL, Wales

[11] Shenzhen Middle Sch, Shenzhen 518001, Guangdong, Peoples R China

[12] Huizhou Second Maternal & Child Hlth Care Hosp, Huizhou 516001, Guangdong, Peoples R China

[13] Shenzhen Engn Res Ctr Med Bioact Mat, Shenzhen 518055, Guangdong, Peoples R China

来源：

JOURNAL OF ORTHOPAEDIC TRANSLATION | 2020年 / 22卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Angiogenesis; Genistein; Rheumatoid arthritis; ENDOTHELIAL GROWTH-FACTOR; VEGF EXPRESSION; CARCINOMA CELLS; STAT3; INTERLEUKIN-6; PROTEIN; IL-6;

D O I：

10.1016/j.jot.2019.07.007

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Background: Angiogenesis plays an important role in the development of rheumatoid arthritis (RA), which increases the supply of nutrients, cytokines, and inflammatory cells to the synovial membrane. Genistein (GEN), a soy-derived isoflavone, has been validated that can effectively inhibit the angiogenesis of several tumours. We thus carried out a study in vitro to investigate the effect of GEN in vascular endothelial growth factor (VEGF) expression and angiogenesis induced by the inflammatory environment of RA. Methods: MH7A cells were used to verify whether GEN can inhibit the expression of VEGF in MH7A cells under inflammatory conditions and demonstrate the mechanism. EA.hy926 cells were used to verify whether GEN can inhibit the migration and tube formation of vascular endothelial cells in inflammatory environment. Results: GEN dose-dependently inhibited the expression and secretion of interleukin (IL)-6 and VEGF, as well as the nucleus translocation of Signal transducer and activator of transcription 3 (STAT3) in MH7A. Furthermore, GEN inhibited IL-6 -induced vascular endothelial cell migration and tube formation in vitro. Conclusion: GEN inhibits IL-6 -induced VEGF expression and angiogenesis partially through the Janus kinase 2 (JAK2)/STAT3 pathway in RA, which has provided a novel insight into the antiangiogenic activity of GEN in RA. The translational potential of this article: Our study provides scientific guidance for the clinical translational research of GEN in the RA treatment.

引用

页码：92 / 100

页数：9

共 36 条

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