A minor groove RNA triple helix within the catalytic core of a group I intron

被引：75

作者：

Szewczak, AA ^{[1
]}

Ortoleva-Donnelly, L ^{[1
]}

Ryder, SP ^{[1
]}

Moncoeur, E ^{[1
]}

Strobel, SA ^{[1
]}

机构：

[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1998年 / 5卷 / 12期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/4146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Close packing of several double helical and single stranded RNA elements is required for the Tetrahymena group I ribozyme to achieve catalysis. The chemical basis of these packing interactions is largely unknown. Using nucleotide analog interference suppression (NAIS), we demonstrate that the PI substrate helix and J8/7 single stranded segment form an extended minor groove triple helix within the catalytic core of the ribozyme, Because each triple in the complex is mediated by at least one 2'-OH group, this substrate recognition triplex is unique to RNA and is fundamentally different from major groove homopurine-homopyrimidine triplexes. We have incorporated these biochemical data into a structural model of the ribozyme core that explains how the J8/7 strand organizes several helices within this complex RNA tertiary structure.

引用

页码：1037 / 1042

页数：6

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