Transfer of sulfur to biotin from biotin synthase (BioB protein)

被引：53

作者：

Gibson, KJ

Pelletier, DA

Turner, IM

机构：

[1] DuPont Co Inc, Expt Stn, Wilmington, DE 19880 USA

[2] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 254卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.9991

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In biotin synthase reactions carried out in vitro, we observed efficient transfer of S-35 to biotin from partially purified E. coli biotin synthase (product of the bioB gene) labelled by biosynthetic incorporation of [S-35]-cysteine. Mass spectrometry was consistent with covalent alteration of the protein in the assay. These results suggest that BioB protein is a reagent, not a catalyst. (C) 1999 Academic Press.

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页码：632 / 635

页数：4

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