A combination of synchrotron and laboratory X-ray techniques for studying tissue-specific trace level metal distributions in Daphnia magna

被引：47

作者：

De Samber, B. ^{[1
]}

Evens, R. ^{[2
]}

De Schamphelaere, K. ^{[2
]}

Silversmit, G. ^{[1
]}

Masschaele, B. ^{[3
]}

Schoonjans, T. ^{[1
]}

Vekemans, B. ^{[1
]}

Janssen, C. R. ^{[2
]}

Van Hoorebeke, L. ^{[3
]}

Szaloki, I. ^{[4
]}

Vanhaecke, F. ^{[1
]}

Falkenberg, G. ^{[5
]}

Vincze, L. ^{[1
]}

机构：

[1] Univ Ghent, Dept Analyt Chem, B-9000 Ghent, Belgium

[2] Univ Ghent, Lab Environm Toxicol & Aquat Ecol, B-9000 Ghent, Belgium

[3] Univ Ghent, Ctr Xray Tomog, Dept Subatom & Radiat Phys, B-9000 Ghent, Belgium

[4] Univ Debrecen, Inst Expt Med, H-4026 Debrecen, Hungary

[5] DESY, Hamburger Synchrotronstrahlungslabor, D-22603 Hamburg, Germany

来源：

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY | 2008年 / 23卷 / 06期

关键词：

D O I：

10.1039/b800343m

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

In the field of environmental toxicology, the study on the effects of the presence of transition metals such as Cu, Ni and Zn on the health of pelagic and benthic invertebrates is an important research topic. Fast dynamic 2D micro-XRF scanning and dynamic XRF micro-CT experiments on D. magna, a frequently used ecotoxicological model organism, allow the quantitative investigation of the accumulation of metals within specific organs with microscopic resolution. K-means clustering allows comparison of the concentrations of the elements of interest between different tissues/organs. Principal component analysis allows most of the significant statistical variations in these large and complex multivariate data sets to be explained. Also, by combining SR-XRF and absorption CT data sets using appropriate software packages, it becomes possible to unravel the tissue-specific 2D/3D distribution of metals in-situ within delicate organic samples on the 3-15 mu m resolution level in an essentially non-destructive manner.

引用

页码：829 / 839

页数：11

共 39 条

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