Detection of potentially pathogenic Aeromonas isolates from ready-to-eat seafood products by PCR analysis

被引:23
作者
Di Pinto, Angela [1 ]
Terio, Valentina [1 ]
Di Pinto, Pietro [1 ]
Tantillo, Giuseppina [1 ]
机构
[1] Univ Bari Aldo Moro, Fac Med Vet, Dipartimento Sanita Pubbl & Zootecnia, I-70010 Bari, Italy
关键词
Aeromonas spp; PCR; ready to eat; seafood; PREVALENCE; HYDROPHILA; DIARRHEA; SPP;
D O I
10.1111/j.1365-2621.2011.02835.x
中图分类号
TS2 [食品工业];
学科分类号
100403 [营养与食品卫生学];
摘要
This study provides data on the prevalence of potentially pathogenic Aeromonas spp. in ready-to-eat (RTE) seafood products by evaluating the occurrence of Aeromonas spp. and the presence of virulence-associated genes. Aeromonas spp. was detected in 57/81 (70.3%) RTE seafood samples. Specifically, Aeromonas spp. was highlighted in 19/21 (90.5%) sushi, in 18/21 (85.7%) sea salad, 11/12 (91.7%) surimi and 9/12 (75%) peeled shrimp samples. Aeromonas spp. was not observed in marinated anchovies and octopus salad samples. Then, PCRs aimed at the hlyA, aerA, alt and ast genes, encoding, respectively, haemolysin A, aerolysin, aeromonas labile temperature cytotonic enterotoxin and aeromonas stable temperature cytotonic enterotoxin, demonstrated a widespread distribution of these genes among Aeromonas isolates. The results underline the need to implement an adequate control plan performing an intensive and continuous monitoring to guarantee the human health.
引用
收藏
页码:269 / 273
页数:5
相关论文
共 20 条
[1]
Prevalence of enterotoxin genes in Aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment [J].
Albert, MJ ;
Ansaruzzaman, M ;
Talukder, KA ;
Chopra, AK ;
Kuhn, I ;
Rahman, M ;
Faruque, ASG ;
Islam, MS ;
Sack, RB ;
Mollby, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (10) :3785-3790
[2]
Comparison of phenotypical and genetic identification of Aeromonas strains isolated from diseased fish [J].
Beaz-Hidalgo, Roxana ;
Alperi, Anabel ;
Bujan, Noemi ;
Romalde, Jesus L. ;
Jose Figueras, Maria .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2010, 33 (03) :149-153
[3]
Detection of potentially enterotoxigenic food-related Bacillus cereus by PCR analysis [J].
Bonerba, Elisabetta ;
Di Pinto, Angela ;
Novello, Lucia ;
Montemurro, Filomena ;
Terio, Valentina ;
Colao, Valeriana ;
Ciccarese, Giuseppina ;
Tantillo, Giuseppina .
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2010, 45 (06) :1310-1315
[4]
Epidemiological Surveillance System, 2007, Boletin Epidemiol Semanal, V15, P109
[5]
Galindo C.L., 2007, AEROMONAS PLESIOMONA, P381
[6]
Galindo Cristi L., 2006, Current Immunology Reviews, V2, P13, DOI 10.2174/157339506775471910
[7]
The Genus Aeromonas: Taxonomy, Pathogenicity, and Infection [J].
Janda, J. Michael ;
Abbott, Sharon L. .
CLINICAL MICROBIOLOGY REVIEWS, 2010, 23 (01) :35-73
[8]
Distribution of Aeromonas spp. as identified by 16S rDNA restriction fragment length polymorphism analysis in a trout farm [J].
Lee, C ;
Cho, JC ;
Lee, SH ;
Lee, DG ;
Kim, SJ .
JOURNAL OF APPLIED MICROBIOLOGY, 2002, 93 (06) :976-985
[9]
Martin-Carnahan A., 2005, BERGEYS MANUAL SYSTE, V2, P557
[10]
NISHIKAWA Y, 1993, INT J FOOD MICROBIOL, V18, P271, DOI 10.1016/0168-1605(93)90150-F