A simple robust method for synthesis of metallic copper nanoparticles of high antibacterial potency against E. coli

被引:174
作者
Chatterjee, Arijit Kumar [1 ]
Sarkar, Raj Kumar [2 ]
Chattopadhyay, Asoke Prasun [2 ]
Aich, Pulakesh [1 ]
Chakraborty, Ruchira [1 ]
Basu, Tarakdas [1 ]
机构
[1] Univ Kalyani, Dept Biochem & Biophys, Kalyani 741235, W Bengal, India
[2] Univ Kalyani, Dept Chem, Kalyani 741235, W Bengal, India
关键词
ESCHERICHIA-COLI; CELL-DIVISION; REVERSE MICELLES; FTSZ; SIZE; SULA; SOS; PARTICLES; PROTEIN; POLYMERIZATION;
D O I
10.1088/0957-4484/23/8/085103
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
A method for preparation of copper nanoparticles (Cu-NPs) was developed by simple reduction of CuCl2 in the presence of gelatin as a stabilizer and without applying stringent conditions like purging with nitrogen. The NPs were characterized by spectrophotometry, dynamic light scattering, x-ray diffraction, transmission electron microscopy, atomic force microscopy and x-ray photoelectron spectroscopy. The particles were about 50-60 nm in size and highly stable. The antibacterial activity of this Cu-NP on Gram-negative Escherichia coli was demonstrated by the methods of agar plating, flow cytometry and phase contrast microscopy. The minimum inhibitory concentration (3.0 mu g ml(-1)), minimum bactericidal concentration (7.5 mu g ml(-1)) and susceptibility constant (0.92) showed that this Cu-NP is highly effective against E. coli at a much lower concentration than that reported previously. Treatment with Cu-NPs made E. coli cells filamentous. The higher the concentration of Cu-NPs, the greater the population of filamentous cells; average filament size varied from 7 to 20 mu m compared to the normal cell size of similar to 2.5 mu m. Both filamentation and killing of cells by Cu-NPs (7.5 mu g ml(-1)) also occurred in an E. coli strain resistant to multiple antibiotics. Moreover, an antibacterial effect of Cu-NPs was also observed in Gram-positive Bacillus subtilis and Staphylococcus aureus, for which the values of minimum inhibitory concentration and minimum bactericidal concentration were close to that for E. coli.
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页数:11
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共 42 条
[1]   Large-scale production of carbon-coated copper nanoparticles for sensor applications [J].
Athanassiou, EK ;
Grass, RN ;
Stark, WJ .
NANOTECHNOLOGY, 2006, 17 (06) :1668-1673
[2]   Synthesis of CTAB-IPA reduced copper nanoparticles [J].
Athawale, AA ;
Katre, PP ;
Kumar, M ;
Majumdar, MB .
MATERIALS CHEMISTRY AND PHYSICS, 2005, 91 (2-3) :507-512
[3]   FTSZ RING STRUCTURE ASSOCIATED WITH DIVISION IN ESCHERICHIA-COLI [J].
BI, E ;
LUTKENHAUS, J .
NATURE, 1991, 354 (6349) :161-164
[4]   Putting copper into action: copper-impregnated products with potent biocidal activities [J].
Borkow, G ;
Gabbay, J .
FASEB JOURNAL, 2004, 18 (12) :1728-+
[5]   FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell division [J].
Chen, JC ;
Beckwith, J .
MOLECULAR MICROBIOLOGY, 2001, 42 (02) :395-413
[6]   The use of CTAB to control the size of copper nanoparticles and the concentration of alkylthiols on their surfaces [J].
Chen, L ;
Zhang, DJ ;
Chen, JM ;
Zhou, HD ;
Wan, HQ .
MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING, 2006, 415 (1-2) :156-161
[7]   Crystal structure of the SOS cell division inhibitor SulA and in complex with FtsZ [J].
Cordell, SC ;
Robinson, EJH ;
Löwe, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (13) :7889-7894
[8]   Aminoclay: a permselective matrix to stabilize copper nanoparticles [J].
Datta, K. K. R. ;
Kulkarni, C. ;
Eswaramoorthy, M. .
CHEMICAL COMMUNICATIONS, 2010, 46 (04) :616-618
[9]   Synthesis, characterization, and properties of metallic copper nanoparticles [J].
Dhas, NA ;
Raj, CP ;
Gedanken, A .
CHEMISTRY OF MATERIALS, 1998, 10 (05) :1446-1452
[10]   Preparation, characterization and antibacterial properties against E-coli K88 of chitosan nanoparticle loaded copper ions [J].
Du, Wen-Li ;
Xu, Ying-Lei ;
Xu, Zi-Rong ;
Fan, Cheng-Li .
NANOTECHNOLOGY, 2008, 19 (08)