Fate map of serotonin transporter-expressing cells in developing mouse heart

被引：22

作者：

Pavone, Luigi Michele ^{[1
,2
]}

Mithbaokar, Pratibha ^{[1
]}

Mastellone, Vincenzo ^{[3
]}

Avallone, Luigi ^{[1
]}

Gaspar, Patricia ^{[4
]}

Maharajan, Veeramani ^{[5
]}

Baidini, Antonio ^{[6
,7
]}

机构：

[1] Univ Naples Federico 2, Dept Biol Struct Funct & Technol, I-80137 Naples, Italy

[2] Univ Naples Federico 2, Dept Biochem & Med Biotechnol, I-80137 Naples, Italy

[3] Magna Graecia Univ Catanzaro, Dept Expt Med G Salvatore, Catanzaro, Italy

[4] INSERM, U616, Paris, France

[5] CNR, Inst Cybernet, I-80125 Naples, Italy

[6] Telethon Inst Genet & Med, Naples, Italy

[7] IBT, Houston, TX USA

来源：

GENESIS | 2007年 / 45卷 / 11期

关键词：

SERT; Cre-recombinase; heart development; serotonergic system; mouse model;

D O I：

10.1002/dvg.20343

中图分类号：

Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Serotonin regulates cardiovascular functions during embryogenesis and adulthood. However, the, source of serotonin in the cardiovascular system and the role of circulating serotonin and serotonin transporter (SERT) in the regulation of cardiovascular functions are still unclear. We used a cell fate approach to map the regions of the mouse heart expressing SERT, utilizing a Cre/loxP system driven by SERT gene expression. Cell labelling was first detected at E10.5 and was mapped until E18.5. We found labelling in the outflow tract, part of right ventricle and to a very limited extent in the left ventricle. Interestingly, the distribution pattern of SERT-fated cells was remarkably similar to that obtained with markers of the second heart field lineage. In addition, we observed staining of atrioventricular valves, consistent with valvular abnormalities observed in SERT-/-animals. Overall, our data reveal specific and regionally restricted distribution of SERT-expressing cells in the developing heart of mouse. genesis 45:689695, 2007. (c) 2007 Wiley-Liss, Inc.

引用

页码：689 / 695

页数：7

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