Changes in paired-pulse facilitation correlate with induction of long-term potentiation in area CA1 of rat hippocampal slices

被引:60
作者
Kleschevnikov, AM
Sokolov, MV
Kuhnt, U
Dawe, GS
Stephenson, JD
Voronin, LL
机构
[1] RUSSIAN ACAD MED SCI, BRAIN RES INST, MOSCOW 103064, RUSSIA
[2] MAX PLANCK INST BIOPHYS CHEM, D-37077 GOTTINGEN, GERMANY
[3] INST PSYCHIAT, LONDON SE5 8AF, ENGLAND
基金
俄罗斯基础研究基金会;
关键词
long-term potentiation; paired-pulse facilitation; field potentials; CA1; hippocampus; rat;
D O I
10.1016/S0306-4522(96)00342-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The phenomenon of long-term potentiation is widely used as an experimental model of memory. An approach that has been used to study its underlying mechanisms is to analyse its interaction with presynaptic paired-pulse Facilitation. Several studies found no evidence for an interaction in the CAl hippocampal area, whereas other data, for example from quantal analysis, suggested that presynaptic mechanisms contribute to the maintenance of long-term potentiation. In the present study, initial slopes of field potentials in area CAl were measured in rat hippocampal slices. ''Conventional'' long-term potentiation was induced by high-frequency (100 Hz) afferent tetanization of the testing input. ''Associative'' long-term potentiation was induced by combining lower frequency (40 Hz) tetanization of a testing input with high-frequency tetanization of a second input. The paired-pulse facilitation ratio decreased in the majority of experiments in which long-term potentiation was induced conventionally, but it decreased, increased or did not change after inducing associative potentiation. Decreases in the paired-pulse facilitation correlated inversely with the initial (pre-tetanic) facilitation ratio. A more detailed regression analysis suggests that this correlation results from two other correlations: (i) that between changes in paired-pulse facilitation and the magnitude of long-term potentiation, and (ii) that between initial paired-pulse facilitation and the magnitude of long-term potentiation. The first correlation prevailed during the initial 10 min following tetanization, while the second prevailed 40-60 min later. A post-tetanic decrease in paired-pulse facilitation is evidence for an involvement of presynaptic mechanisms in the maintenance of long-term potentiation. The lack of significant changes in some studies could be due to the inclusion in the analyses of experiments with long-term potentiation of small magnitude, in which changes in paired-pulse facilitation ratios would have beer, inconsistent. The present study suggests that the early (10-20 min) and late (40-50 min) phases of long-term potentiation were mediated by different mechanisms, with a mixture of these mechanisms during the intermediate period. On the basis of the present and previous studies, the following scheme of involvement of several mechanisms in long-term potentiation maintenance is proposed. The early phase includes two major mechanisms: an increase in the probability of transmitter release, leading to an apparent increase in the number of effective release sites, and an increase in efficacy of one transmitter quantum, probably due to an increased number of postsynaptic receptors. The later phase of long-term potentiation is attributed to an increase in the number of transmitter zones, presumably due to structural modifications. Copyright (C) 1996 IBRO.
引用
收藏
页码:829 / 843
页数:15
相关论文
共 81 条
  • [1] Andersen P, 1967, Prog Brain Res, V27, P400
  • [2] ANDERSEN P, 1971, EXP BRAIN RES, V13, P208
  • [3] ORIGINS OF THE VARIATIONS IN LONG-TERM POTENTIATION BETWEEN SYNAPSES IN THE BASAL VERSUS APICAL DENDRITES OF HIPPOCAMPAL-NEURONS
    ARAI, A
    BLACK, J
    LYNCH, G
    [J]. HIPPOCAMPUS, 1994, 4 (01) : 1 - 9
  • [4] ENHANCED SENSITIVITY OF METABOTROPIC GLUTAMATE RECEPTORS AFTER INDUCTION OF LONG-TERM POTENTIATION IN RAT HIPPOCAMPUS
    ARONICA, E
    FREY, U
    WAGNER, M
    SCHROEDER, H
    KRUG, M
    RUTHRICH, H
    CATANIA, MV
    NICOLETTI, F
    REYMANN, KG
    [J]. JOURNAL OF NEUROCHEMISTRY, 1991, 57 (02) : 376 - 383
  • [5] Atwood H L, 1976, Prog Neurobiol, V7, P291, DOI 10.1016/0301-0082(76)90009-5
  • [6] BACKHAUS K, 1980, MULTIVARIATE ANALYSE
  • [7] STRUCTURAL-CHANGES ACCOMPANYING MEMORY STORAGE
    BAILEY, CH
    KANDEL, ER
    [J]. ANNUAL REVIEW OF PHYSIOLOGY, 1993, 55 : 397 - 426
  • [8] ASSOCIATIVE LONG-TERM POTENTIATION IN HIPPOCAMPAL SLICES
    BARRIONUEVO, G
    BROWN, TH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (23): : 7347 - 7351
  • [9] BASKYS A, 1978, DOKL ACAD SCI USSR, V239, P234
  • [10] A SYNAPTIC MODEL OF MEMORY - LONG-TERM POTENTIATION IN THE HIPPOCAMPUS
    BLISS, TVP
    COLLINGRIDGE, GL
    [J]. NATURE, 1993, 361 (6407) : 31 - 39