P42/p44 mitogen-activated protein kinase activation is involved in prostaglandin F2α-induced interleukin-6 synthesis in osteoblasts

被引：27

作者：

Tokuda, H ^{[1
]}

Kozawa, O

Harada, A

Uematsu, T

机构：

[1] Chubu Natl Hosp, Natl Inst Longev Sci, Dept Internal Med, Obu 4748511, Japan

[2] Chubu Natl Hosp, Natl Inst Longev Sci, Dept Orthopaed, Obu 4748511, Japan

[3] Gifu Univ, Sch Med, Dept Pharmacol, Gifu 5008076, Japan

来源：

CELLULAR SIGNALLING | 1999年 / 11卷 / 05期

关键词：

prostaglandin F-2 alpha; interleukin-6; p42/p44 MAP kinase; protein kinase C; osteoblast;

D O I：

10.1016/S0898-6568(98)00048-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Prostaglandin F-2 alpha (PGF(2 alpha)) significantly induced p42/p44 mitogen-activated protein (MAP) kinase activity in osteoblast-like MC3T3-E1 cells. PD98059, a selective inhibitor of MAP kinase kinase, inhibited PGF(2 alpha)-induced interleukin-6 (IL-6) synthesis as well as PGF(2 alpha)-induced p42/p44 MAP kinase activation. PD98059 suppressed the IL-6 synthesis induced by 12-O-tetradecanoylphorbol 13-acetate (TPA), a protein kinase C (PKC) activator, or NaF, an activator of heterotrimeric GTP-binding protein, as well as the p42/p44 MAP kinase activation by TPA or NaF. Calphostin C, a highly potent and specific inhibitor of PKC, inhibited the PGF(2 alpha)-induced p42/p44 MAP kinase activity, These results strongly suggest that PKC-dependent p42/p44 MAP kinase activation is involved in PGF(2 alpha)-induced IL-6 synthesis in osteoblasts. CELL SIGNAL 11;5:325-330, 1999. (C) 1999 Elsevier Science Inc.

引用

页码：325 / 330

页数：6

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