Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)

被引：5

作者：

Son, R ^{[1
]}

Nasreldin, EH

Zaiton, H

Samuel, L

Rusul, G

Nimita, F

机构：

[1] Univ Putra Malaysia, Fac Food Sci & Biotechnol, Dept Biotechnol, Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Fac Food Sci & Biotechnol, Dept Food Sci, Serdang 43400, Selangor, Malaysia

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1998年 / 14卷 / 06期

关键词：

V-parahaemolyticus; plasmid; RAPD; cockles (Anadara granosa);

D O I：

10.1023/A:1008841514368

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3 kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio pal parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus.

引用

页码：895 / 901

页数：7

共 26 条

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