Detection of homocysteine and cysteine

被引：558

作者：

Wang, WH ^{[1
]}

Rusin, O ^{[1
]}

Xu, XY ^{[1
]}

Kim, KK ^{[1
]}

Escobedo, JO ^{[1
]}

Fakayode, SO ^{[1
]}

Fletcher, KA ^{[1
]}

Lowry, M ^{[1
]}

Schowalter, CM ^{[1
]}

Lawrence, CM ^{[1
]}

Fronczek, FR ^{[1
]}

Warner, IM ^{[1
]}

Strongin, RM ^{[1
]}

机构：

[1] Louisiana State Univ, Dept Chem, Baton Rouge, LA 70803 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 45期

关键词：

D O I：

10.1021/ja054962n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

At elevated levels, homocysteine (Hey, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hey or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hey in the range of their physiologically relevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potential biomedical relevance of the chemical mechanisms involved in the detection of 1 is described.

引用

页码：15949 / 15958

页数：10

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