The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450CYP83B1, a modulator of auxin homeostasis

被引:227
作者
Barlier, I
Kowalczyk, M
Marchant, A
Ljung, K
Bhalerao, R
Bennett, M
Sandberg, G
Bellini, C
机构
[1] Biol Cellulaire Lab, F-78026 Versailles, France
[2] Swedish Univ Agr Sci, Dept Forest Genet & Plant Physiol, S-90183 Umea, Sweden
[3] Univ Nottingham, Sch Biosci, Nottingham NG7 2RD, England
关键词
metabolism;
D O I
10.1073/pnas.260502697
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Genetic screens have been performed to identify mutants with altered auxin homeostasis in Arabidopsis. A tagged allele of the auxin-overproducing mutant sur2 was identified within a transposon mutagenized population. The SUR2 gene was cloned and shown to encode the CYP83B1 protein, which belongs to the targe family of the P450-dependent monooxygenases. SUR2 expression is up-regulated in sur1 mutants and induced by exogenous auxin in the wild type. Analysis of indole3-acetic acid (IAA) synthesis and metabolism in sur2 plants indicates that the mutation causes a conditional increase in the pool size of IAA through up-regulation of IAA synthesis.
引用
收藏
页码:14819 / 14824
页数:6
相关论文
共 32 条
[1]   TRANSPOSON TAGGING OF A MALE-STERILITY GENE IN ARABIDOPSIS [J].
AARTS, MGM ;
DIRKSE, WG ;
STIEKEMA, WJ ;
PEREIRA, A .
NATURE, 1993, 363 (6431) :715-717
[2]  
Bartel Bonnie, 1997, Annu Rev Plant Physiol Plant Mol Biol, V48, P51, DOI 10.1146/annurev.arplant.48.1.51
[3]   Successful PCR-based reverse genetic screens using an En-1-mutagenised Arabidopsis thaliana population generated via single-seed descent [J].
Baumann, E ;
Lewald, J ;
Saedler, H ;
Schulz, B ;
Wisman, E .
THEORETICAL AND APPLIED GENETICS, 1998, 97 (5-6) :729-734
[4]  
BENNETT M, 1998, ARABIDOPSIS, P107
[5]  
BOERJAN W, 1995, PLANT CELL, V7, P1405, DOI 10.1105/tpc.7.9.1405
[6]   The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22α-hydroxylation steps in brassinosteroid biosynthesis [J].
Choe, SW ;
Dilkes, BP ;
Fujioka, S ;
Takatsuto, S ;
Sakurai, A ;
Feldmann, KA .
PLANT CELL, 1998, 10 (02) :231-243
[7]  
Davies Peter J., 1995, P1
[8]   Sur2 mutations of Arabidopsis thaliana define a new locus involved in the control of auxin homeostasis [J].
Delarue, M ;
Prinsen, E ;
Van Onckelen, H ;
Caboche, M ;
Bellini, C .
PLANT JOURNAL, 1998, 14 (05) :603-611
[9]  
Doyle J. L. ., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362
[10]  
Durst Francis, 1995, Drug Metabolism and Drug Interactions, V12, P171