2D or not 2D

被引:138
作者
Fey, SJ [1 ]
Larsen, PM [1 ]
机构
[1] Univ So Denmark, Ctr Proteome Anal, DK-5230 Odense M, Denmark
关键词
D O I
10.1016/S1367-5931(00)00167-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
2D gel electrophoresis is the technology that everyone loves to hate - it requires manual dexterity and precision to reproduce precisely and is thus not well-suited as a high-throughput technology. Although almost everyone would like to replace it, the resolution and sensitivity it offers are exquisite and unsurpassed if one wants a global view of cellular activity. There have been several recent developments, for example, the detection of low abundance proteins, and the resolution possible with narrow-range IPG gels.
引用
收藏
页码:26 / 33
页数:8
相关论文
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