Icariin stimulates osteogenic differentiation and suppresses adipogenic differentiation of rBMSCs via estrogen receptor signaling

被引：43

作者：

Li, Xiaoyun ^{[1
]}

Peng, Bojia ^{[1
]}

Pan, Yanbin ^{[1
]}

Wang, Panpan ^{[1
]}

Sun, Kehuan ^{[2
]}

Lei, Xiaotong ^{[1
]}

Ou, Ling ^{[1
]}

Wu, Zhidi ^{[1
]}

Liu, Xiaoguang ^{[2
]}

Wang, Haixia ^{[2
]}

He, Haibin ^{[1
]}

Mo, Shu ^{[2
]}

Tian, Ya ^{[1
]}

Peng, Xunqian ^{[2
]}

Zhu, Xiaofeng ^{[3
]}

Zhang, Ronghua ^{[1
]}

Yang, Li ^{[1
]}

机构：

[1] Jinan Univ, Dept Tradit Chinese Pharmacol, Coll Pharm, 601 Huangpu Ave West, Guangzhou 510632, Guangdong, Peoples R China

[2] Jinan Univ, Coll Tradit Chinese Med, Guangzhou 510632, Guangdong, Peoples R China

[3] Jinan Univ, Dept Affiliated Hosp 1, Guangzhou 510630, Guangdong, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2018年 / 18卷 / 03期

基金：

中国国家自然科学基金;

关键词：

icariin; estrogen receptor signaling; osteogenic differentiation; adipogenic differentiation; MESENCHYMAL STEM-CELLS; BONE-MARROW; GENE-EXPRESSION; OSTEOPOROSIS; BISPHOSPHONATES; INCREASES; PATHWAY; TISSUE; ALPHA;

D O I：

10.3892/mmr.2018.9325

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Icariin (ICA) is a major active ingredient in Herba epimedii, which is commonly used as a Chinese herbal medicine for the treatment of osteoporosis. Previous studies have revealed that ICA exerted a protective effect against bone loss and increased bone regeneration; however, the association between ICA and estrogen receptor (ER) signaling remains unclear. The aim of the present study was to determine the effect of ICA on rat bone marrow stromal cells (rBMSCs). Cell Counting Kit-8 assays were conducted to measure proliferation, alkaline phosphatase (ALP) activity was evaluated to assess osteoblast differentiation, and reverse transcription-quantitative polymerase chain reaction as well as western blotting were performed to detect the expression of cellular and molecular markers of osteogenic or adipogenic differentiation. The results demonstrated that treatment of rBMSCs with 10(-6) M ICA stimulated rBMSC proliferation and ALP activity. Furthermore, ICA treatment increased the expression of the osteogenic markers runt-related transcription factor 2, collagen type 1 and bone morphogenetic Protein 2; however, it also decreased the expression of the adipogenic differentiation markers peroxisome proliferator-activated receptor gamma and CCAAT/enhancer-binding protein alpha. Treatment of rBMSCs with ICI182780, an ER antagonist, blocked the effects of ICA. Taken together, these findings indicated that ICA may stimulate osteoblast differentiation and inhibit adipogenic differentiation via the activation of the ER signaling pathway. Therefore, ICA has the potential to serve as a therapeutic alternative for the prevention and treatment of osteoporosis.

引用

页码：3483 / 3489

页数：7

共 27 条

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miR-17, miR-21, and miR-143 Enhance Adipogenic Differentiation from Porcine Bone Marrow-Derived Mesenchymal Stem Cells [J].