Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea

被引:25
作者
Cruchaga, Saioa [2 ]
Artola, Ekhine [2 ]
Lasa, Berta [2 ]
Ariz, Idoia [1 ]
Irigoyen, Ignacio [3 ]
Fernando Moran, Jose [1 ]
Aparicio-Tejo, Pedro M. [1 ]
机构
[1] UPNa CSIC GN, Inst Agribiotechnol Inst IdAB, Navarra 31192, Spain
[2] Univ Publ Navarra, Dpto Ciencias Medio Nat, Navarra 31600, Spain
[3] Univ Publ Navarra, Dpto Prod Agr, Navarra 31600, Spain
关键词
Ammonium; N-(n-butyl) thiophosphoric triamide (NBPT); Urease inhibitor; Urea; N-(N-BUTYL) THIOPHOSPHORIC TRIAMIDE; INDUCED FLUORESCENCE DETECTION; AMINO-ACIDS; CAPILLARY-ELECTROPHORESIS; INSECTICIDAL PROPERTIES; AMMONIA VOLATILIZATION; MICROBIAL UREASES; NITROGEN; PLANTS; PHYTOTOXICITY;
D O I
10.1016/j.jplph.2010.07.024
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The application of urease inhibitors in conjunction with urea fertilizers as a means of reducing N loss due to ammonia volatilization requires an in-depth study of the physiological effects of these inhibitors on plants. The aim of this study was to determine how the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) affects N metabolism in pea and spinach. Plants were cultivated in pure hydroponic culture with urea as the sole N source. After 2 weeks of growth for pea, and 3 weeks for spinach, half of the plants received NBPT in their nutrient solution. Urease activity, urea and ammonium content, free amino acid composition and soluble protein were determined in leaves and roots at days 0, 1, 2, 4, 7 and 9, and the NBPT content in these tissues was determined 48 h after inhibitor application. The results suggest that the effects of NBPT on spinach and pea urease activity differ, with pea being most affected by this treatment, and that the NBPT absorbed by the plant caused a clear inhibition of the urease activity in pea leaf and roots. The high urea concentration observed in leaves was associated with the development of necrotic leaf margins, and was further evidence of NBPT inhibition in these plants. A decrease in the ammonium content in roots, where N assimilation mainly takes place, was also observed. Consequently, total amino acid contents were drastically reduced upon NBPT treatment, indicating a strong alteration of the N metabolism. Furthermore, the amino acid profile showed that amidic amino acids were major components of the reduced pool of amino acids. In contrast, NBPT was absorbed to a much lesser degree by spinach plants than pea plants (35% less) and did not produce a clear inhibition of urease activity in this species. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码:329 / 336
页数:8
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