Ursolic acid regulates aging process through enhancing of metabolic sensor proteins level

We previously reported that Ursolic Acid (UA) ameliorates skeletal muscle performance through satellite cells proliferation and cellular energy status. In studying the potential role of the hypothalamus in aging, we developed a strategy to pursue UA effects on the hypothalamus anti-aging proteins such as; SIRT1, SIRT6, PGC-1 beta and alpha-Klotho. In this study, we used a model of aging animals (C57BL/6). UA dissolved in Corn oil (20 mg/ml) and then administrated (200 mg/Kg i.p injection) to mice, twice daily for 7 days. After treatment times, the mice perfused and the hypothalamus isolated for preparing of tissue to Immunofluorescence microscopy. The data illustrated that UA significantly increased SIRT1 (similar to 3.5 +/- 0.3 folds) and SIRT-6 (similar to 1.5 +/- 0.2 folds) proteins overexpression (P < 0.001). In addition, our results showed that UA enhanced alpha-Klotho (similar to 3.3 +/- 0.3) and PGC-1 beta (similar to 2.6 +/- 0.2 folds) proteins levels (P < 0.01). In this study, data were analyzed using SPSS 16 (ANOVA test). To the best of our knowledge, it seems that UA through enhancing of anti-aging biomarkers (SIRT1 and SIRT6) and PGC-1 beta in hypothalamus regulates aging-process and attenuates mitochondrial-related diseases. In regard to the key role of alpha-Klotho in aging, our data indicate that UA may be on the horizon to forestall diseases of aging. (C) 2016 Elsevier Masson SAS. All rights reserved.