RAD51C is required for Holliday junction processing in mammalian cells

被引：239

作者：

Liu, YL ^{[1
]}

Masson, JY ^{[1
]}

Shah, R ^{[1
]}

O'Regan, P ^{[1
]}

West, SC ^{[1
]}

机构：

[1] Imperial Canc Res Fund, Clare Hall Labs, Canc Res UK, S Mimms EN6 3LD, Herts, England

来源：

SCIENCE | 2004年 / 303卷 / 5655期

关键词：

D O I：

10.1126/science.1093037

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

During genetic recombination and the recombinational repair of chromosome breaks, DNA molecules become linked at points of strand exchange. Branch migration and resolution of these crossovers, or Holliday junctions (HJs), complete the recombination process. Here, we show that extracts from cells carrying mutations in the recombination/repair genes RAD51C or XRCC3 have reduced levels of HJ resolvase activity. Moreover, depletion of RAD51C from fractionated human extracts caused a loss of branch migration and resolution activity, but these functions were restored by complementation with a variety of RAD51 paralog complexes containing RAD51C. We conclude that the RAD51 paralogs are involved in HJ processing in human cells.

引用

页码：243 / 246

页数：4

共 25 条

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