Resveratrol prevents dexamethasone-induced expression of the muscle atrophy-related ubiquitin ligases atrogin-1 and MuRF1 in cultured myotubes through a SIRT1-dependent mechanism

被引：80

作者：

Alamdari, Nima ^{[1
]}

Aversa, Zaira ^{[1
]}

Castillero, Estibaliz ^{[1
]}

Gurav, Aniket ^{[1
]}

Petkova, Victoria ^{[2
]}

Tizio, Steven ^{[1
]}

Hasselgren, Per-Olof ^{[1
]}

机构：

[1] Harvard Univ, Beth Israel Deaconess Med Ctr, Dept Surg, Sch Med, Boston, MA 02215 USA

[2] Harvard Univ, Beth Israel Deaconess Med Ctr, Dept Med, Sch Med, Boston, MA 02215 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 417卷 / 01期

关键词：

Muscle wasting; Glucocorticoids; Acetylation; Deacetylation; Resveratrol; SKELETAL-MUSCLE; FORKHEAD TRANSCRIPTION; MITOCHONDRIAL-FUNCTION; UNCOUPLING PROTEIN-3; METABOLIC-CONTROL; INDUCED MYOPATHY; CANCER CACHEXIA; GENE-EXPRESSION; C2C12; MYOTUBES; C/EBP-BETA;

D O I：

10.1016/j.bbrc.2011.11.154

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Resveratrol (3,5,4'-trihydroxystilbene) has been ascribed multiple beneficial biological effects but the influence of resveratrol on glucocorticoid-induced muscle atrophy is not known. We examined the effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression, FOXO1 acetylation, protein degradation and atrophy in cultured L6 myotubes. In addition, the role of the deacetylase SIRT1 in the effects of resveratrol was determined by transfecting myotubes with SIRT1 siRNA. The catabolic effects of dexamethasone were prevented by resveratrol and the protective effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression were abolished in myotubes transfected with SIRT1 siRNA. Results suggest that resveratrol can prevent glucocorticoid-induced muscle wasting and that this effect is at least in part SIRT1-dependent. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：528 / 533

页数：6

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