Q-Gene: processing quantitative real-time RT-PCR data

被引：713

作者：

Simon, P ^{[1
]}

机构：

[1] Univ Eye Hosp Tuebingen, Sect Neurobiol Eye, D-72076 Tubingen, Germany

来源：

BIOINFORMATICS | 2003年 / 19卷 / 11期

关键词：

D O I：

10.1093/bioinformatics/btg157

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Q-Gene is an application for the processing of quantitative real-time RT-PCR data. It offers the user the possibility to freely choose between two principally different procedures to calculate normalized gene expressions as either means of Normalized Expressions or Mean Normalized Expressions. In this contribution it will be shown that the calculation of Mean Normalized Expressions has to be used for processing simplex PCR data, while multiplex PCR data should preferably be processed by calculating Normalized Expressions. The two procedures, which are currently in widespread use and regarded as more or less equivalent alternatives, should therefore specifically be applied according to the quantification procedure used.

引用

页码：1439 / 1440

页数：2

共 5 条

[1] Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems [J].

Bustin, SA .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2002, 29 (01) :23-39

[2]

Muller PY, 2002, BIOTECHNIQUES, V32, P1372

[3] Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR -: art. no. e36 [J].

Pfaffl, MW ;

Horgan, GW ;

Dempfle, L .

NUCLEIC ACIDS RESEARCH, 2002, 30 (09)

[4]

Vu H L, 2000, Nucleic Acids Res, V28, pE18, DOI 10.1093/nar/28.7.e18

[5] A technique whose time has come [J].

Walker, NJ .

SCIENCE, 2002, 296 (5567) :557-+

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