A new repetitive DNA sequence family in the olive (Olea europaea L.)

被引:7
作者
Lorite, P [1 ]
García, MF [1 ]
Carrillo, JA [1 ]
Palomeque, T [1 ]
机构
[1] Univ Jaen, Dept Biol Expt, Area Genet, Jaen, Spain
来源
HEREDITAS | 2001年 / 134卷 / 01期
关键词
D O I
10.1111/j.1601-5223.2001.00073.x
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Two families of repeated DNA sequences were cloned from Olea europaea ssp sativa cv. "Picual". The first repetitive DNA is organized in a tandem repeat of monomers of 178 bp. Sequencing of several clones showed that it is relatively A-T rich (54.49 %) and possesses short direct and inverted subrepeats as well as some palindromic sequences. Comparison between the monomers revealed heterogeneity of the sequence primary structure. This repetitive DNA is present in several cultivars of olive cultivates. Comparison of sequences with other repetitive DNAs described in Olea europaea has been carried out. No significant similarity was found. All the obtained results suggest that this repetitive DNA described here is a new family of repetitive DNA. The second repetitive DNA is organized in a tandem repeat of monomers of 78 bp. This second family of repetitive DNA showed significant similarity with other repetitive DNAs previously described in Olea europaea. Their existence in new cultivars or olive is shown.
引用
收藏
页码:73 / 78
页数:6
相关论文
共 34 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[3]   Chloroplast-DNA variation in the wild and cultivated olives (Olea europaea L.) of Morocco [J].
Amane, M ;
Ouazzani, N ;
Lumaret, R ;
Debain, C .
EUPHYTICA, 2000, 116 (01) :59-64
[4]   Olive genetic diversity assessed using amplified fragment length polymorphisms [J].
Angiolillo, A ;
Mencuccini, M ;
Baldoni, L .
THEORETICAL AND APPLIED GENETICS, 1999, 98 (3-4) :411-421
[5]   RYE HETEROCHROMATIN .1. STUDIES ON CLUSTERS OF THE MAJOR REPEATING SEQUENCE AND THE IDENTIFICATION OF A NEW DISPERSED REPETITIVE SEQUENCE ELEMENT [J].
APPELS, R ;
MORAN, LB ;
GUSTAFSON, JP .
CANADIAN JOURNAL OF GENETICS AND CYTOLOGY, 1986, 28 (05) :645-657
[6]  
Barranco D, 1985, OLIVAE, V9, P16
[7]   Cytoplasmic male sterility in the olive (Olea europaea L.) [J].
Besnard, G ;
Khadari, B ;
Villemur, P ;
Bervillé, A .
THEORETICAL AND APPLIED GENETICS, 2000, 100 (07) :1018-1024
[8]   Multiple origins for Mediterranean olive (Olea europaea L-ssp europaea) based upon mitochondrial DNA polymorphisms [J].
Besnard, G ;
Bervillé, A .
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 2000, 323 (02) :173-181
[9]   Amount and organization of the heterochromatin in Olea europaea and related species [J].
Bitonti M.B. ;
Cozza R. ;
Chiappetta A. ;
Contento A. ;
Minelli S. ;
Ceccarelli M. ;
Gelati M.T. ;
Maggini F. ;
Baldoni L. ;
Cionini P.G. .
Heredity, 1999, 83 (2) :188-195
[10]   DNA fingerprinting and classification of geographically related genotypes of olive-tree (Olea europaea L.) [J].
Claros, MG ;
Crespillo, R ;
Aguilar, ML ;
Cánovas, FM .
EUPHYTICA, 2000, 116 (02) :131-142