Fibronectin and fibrinogen contribute to the enhanced binding of Staphylococcus aureus to atopic skin

被引:201
作者
Cho, SH
Strickland, I
Boguniewicz, M
Leung, DYM
机构
[1] Natl Jewish Med & Res Ctr, Dept Pediat, Denver, CO 80206 USA
[2] Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80262 USA
关键词
atopic dermatitis; collagen; fibrinogen; fibronectin; Staphylococcus aureus;
D O I
10.1067/mai.2001.117455
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Staphylococcus aureus colonizes the skin lesions of more than 90% of patients with atopic dermatitis (AD). The mechanism for increased S aureas colonization in AD is unknown. However, the initial event in colonization requires adherence of S aureus to the skin. Objective: The purpose of this study was to examine the roles of various bacterial adhesins on S aureus binding to AD skin. Methods: In an attempt to delineate the mechanism behind this adherence process, an in vitro bacterial binding assay was developed to quantitate the adherence of various S aureus strains to AD, psoriatic, and normal skin sections. S aureus strains used in this study were obtained either from cultures of AD skin lesions or from genetically manipulated strains of S aureus that lacked specific microbial surface components recognizing adhesive matrix molecules (MSCRAMMs)-namely, fibronectin-binding protein (Fnbp), fibrinogen-binding protein (CIf), collagen-binding protein (Cna), and their parent strains. In addition, S aureus strains from patients with AD were pretreated with fibronectin or fibrinogen to block MSCRAMM receptors and interfere with binding. Results: Under all experimental conditions, binding of S aureus was localized primarily to the stratum corneum. Immunocytochemical staining of AD skin sections showed a redistribution of fibronectin to the cornified layer, an observation not seen in normal skin. S aureus binding to uninvolved AD skin was significantly greater than the binding to uninvolved psoriatic skin (P <.0001) and normal skin (P <.0005). The Fnbp-negative S aureus showed a significant reduction in binding to the AD skin (P <.0001) but not to the psoriatic and normal skin. In the AD skin, a significant reduction in the binding of S aureus was also observed in the Clf-negative strain (P <.0001) but not in the Cna-negative S aureus. Preincubation of S aureus with either fibronectin or fibrinogen also inhibited bacterial binding to AD skin (P <.0001). Conclusion: These data suggest that fibronectin and fibrinogen-but not collagen-play a major role in the enhanced binding of S aureus to the skin of patients with AD.
引用
收藏
页码:269 / 274
页数:6
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