Protective effects of polydatin against CCl4- induced injury to primarily cultured rat hepatocytes

AIM To investigate the protective effects of polydatin (PD) against injury to primarily cultured rat hepatocytes induced by CCl4. METHODS Rat hepatocytes were separated by methods of liver infusion in vivo and cultured medium (7.5 x 10(5) cells/ mL). Two mL or 0.2 mL was added into 24-well or 96-well plates respectively. Twenty-four hours after cell preculture, PD at concentrations of 10(-7) mol/L - 10(-4) mol/ L was added into each plate. At the same time injury to hepatocytes was induced by adding 10 mmol/ L CCl4. Then, 0.1 mL or 1 mL culture solution was removed from the 96-well or 24-well plates at 6 h, 12 h, 24 h and 48 h after CCl14 intoxication respectively for the determination of OPT, GSH and MDA. At 48 h, the survivability of rat hepatocytes was assayed by the MTT color-metric method. RESULTS After CCl4 challenge, the release of GPT and the formation of MDA in rat hepatocytes markedly increased and maintained at a high level in 48 h, whereas PD with different concentrations could markedly inhibit this elevation with 10(-5) mol/ L PD having the strongest effects and inhibiting rate was over 50%. PD could also improve the decreased content of GSH caused by CCl4 in accordance with the doses used. CCl4 evidently decreased the hepatocyte survivability from 91.0% +/- 7.9% to 35.4% +/- 3.8%. On the other hand, PD at 10(-7) mol/ L - 10(-4) mol/ L could reverse this change and improve the cell survival rates to 56.1% +/- 5.2%, 65.8% +/- 5.0%, 88.7% +/- 6.8% and 75.2% +/- 7.3%, respectively. CONCLUSION PD at 10(-7) mol/ L-10(-4) mol/ L could protect primarily cultured rat hepatocytes against CCl4-induced injury.