High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes

被引：47

作者：

Goto-Inoue, Naoko ^{[1
]}

Hayasaka, Takahiro ^{[1
]}

Sugiura, Yuki ^{[2
,3
]}

Taki, Takao ^{[4
]}

Li, Yu-Teh ^{[5
]}

Matsumoto, Mineo ^{[3
]}

Setou, Mitsutoshi ^{[1
,2
,3
]}

机构：

[1] Hamamatsu Univ Sch Med, Dept Mol Anat, Higashi Ku, Hamamatsu, Shizuoka 4313192, Japan

[2] Tokyo Inst Technol, Dept Biosci & Biotechnol, Kanagawa 2268501, Japan

[3] Mitsubishi Kagaku Inst Life Sci MITILS, Tokyo 1948511, Japan

[4] Otsuka Pharmaceut Co Ltd, Inst Biomed Innovat, Kawaguchi, Tokushima 7710192, Japan

[5] Tulane Univ, Dept Biochem, Sch Med, Hlth Sci Ctr, New Orleans, LA 70112 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 870卷 / 01期

关键词：

thin-layer chromatography; matrix-assisted laser desorption/ionization; glycosphingolipid;

D O I：

10.1016/j.jchromb.2008.06.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. He re we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol). (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：74 / 83

页数：10

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