Isolation of the ATP-binding human homolog of the arsA component of the bacterial arsenite transporter

被引：35

作者：

KurdiHaidar, B ^{[1
]}

Aebi, S ^{[1
]}

Heath, D ^{[1
]}

Enns, RE ^{[1
]}

Naredi, P ^{[1
]}

Hom, DK ^{[1
]}

Howell, SB ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,CTR CANC,LA JOLLA,CA 92093

来源：

GENOMICS | 1996年 / 36卷 / 03期

关键词：

D O I：

10.1006/geno.1996.0494

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Arsenite resistance in bacteria is mediated by an efflux pump composed of the arsA and arsB gene products. We have isolated the human homolog of the bacterial arsA (hARSA-I), a member of the ATPase superfamily with no transmembrane domain. Southern and Northern analyses indicated the presence of two cross-hybridizing genes in the human genome and expression of hARSA-I in many tissues. A rabbit antiserum raised against a glutathione-S-transferase (GST)/hARSA-I fusion protein identified two cross-reacting proteins of 37 and 42 kDa by Western analysis in two different human cell lines. Overexpression of hARSA-I in the embryonal human kidney 293 cell line was accompanied by overproduction of the 37-kDa protein. Biochemical analysis using the GST/hARSA-I fusion protein indicated that hARSA-I is an ATPase analogous to the bacterial ArsA. Thus, hARSA-I is a new eukaryotic member of a highly conserved ATP-binding superfamily of proteins. (C) 1996 Academic Press, Inc.

引用

页码：486 / 491

页数：6

共 18 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] ARSENIC EFFLUX GOVERNED BY THE ARSENIC RESISTANCE DETERMINANT OF STAPHYLOCOCCUS-AUREUS PLASMID PI258 [J].