Characterization of hemicellulases and cellulases produced by Ceriporiopsis subvermispora grown on wood under biopulping conditions

被引：38

作者：

Heidorne, FO ^{[1
]}

Magalhaes, PO ^{[1
]}

Ferraz, AL ^{[1
]}

Milagres, AMF ^{[1
]}

机构：

[1] FAENQUIL, Fac Engn Quim Lorena, Dept Biotechnol, BR-12600970 Lorena, SP, Brazil

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 38卷 / 3-4期

基金：

巴西圣保罗研究基金会;

关键词：

Ceriporiopsis subvermispora; cellulases; electrophoresis; solid state cultivation; xylanases;

D O I：

10.1016/j.enzmictec.2005.06.015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Production of cellulases and hemicellulases by Ceriporiopsis subvermispora cultured on wood chips of Eucalyptus grandis and Pinus taeda was studied. Xylanase, endo-1,4 beta-glucanase, marmanase, beta-glucosidase and beta-xylosidase were detected in enzymatic extracts obtained after 30 days of cultivation. R taeda gave rise to higher enzyme production compared to E. grandis. Biochemical characteristics of cellulases and hemicellulases produced in both wood species were almost the same. The optimum pH for the enzymes was between 4.0 and 5.0 and the optimum temperature was 60 degrees C. Thermal stability of the enzymes at pH 5.0 was appreciable up to 50 degrees C. The profile of proteins contained in extracts recovered from both biotreated wood species showed a similar number of bands during SDS-PAGE electrophoresis. Five bands with molecular weight ranging from 50 to 85 kDa and two bands with high molecular weight (100-200 kDa) were observed. Activity staining for xylanases indicated two enzymes with molecular weight of 80 and 33 kDa. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：436 / 442

页数：7

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