SHED Differentiate into Functional Odontoblasts and Endothelium

被引:362
作者
Sakai, V. T. [1 ,2 ]
Zhang, Z. [1 ]
Dong, Z. [1 ]
Neiva, K. G. [1 ]
Machado, M. A. A. M. [2 ]
Shi, S. [3 ]
Santos, C. F. [4 ]
Noer, J. E. [1 ,5 ,6 ]
机构
[1] Univ Michigan, Sch Dent, Angiogenesis Res Lab, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA
[2] Univ Sao Paulo, Dept Pediat Dent Orthodont & Community Hlth, Bauru Sch Dent, BR-05508 Sao Paulo, Brazil
[3] Univ So Calif, Sch Dent, Ctr Craniofacial Mol Biol, Los Angeles, CA 90089 USA
[4] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Sao Paulo, Brazil
[5] Univ Michigan, Coll Engn, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[6] Univ Michigan, Sch Med, Dept Otolaryngol, Ann Arbor, MI 48109 USA
关键词
endodontics; stem cells; tissue engineering; odontogenesis; angiogenesis; EXFOLIATED DECIDUOUS TEETH; ANGIOGENIC GROWTH-FACTORS; EMBRYONIC STEM-CELLS; DENTAL-PULP; IN-VITRO; GENE; REGENERATION; ACTIVATION; EXPRESSION; RECEPTORS;
D O I
10.1177/0022034510368647
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Studies on mechanisms underlying the differentiation of dental pulp stem cells are critical for the understanding of the biology of odontogenesis and for dental tissue engineering. Here, we tested the hypothesis that stem cells from exfoliated deciduous teeth (SHED) differentiate into functional odontoblasts and endothelial cells. SHED were seeded in tooth slice/scaffolds and implanted subcutaneously into immunodeficient mice. SHED differentiated into functional odontoblasts that generated tubular dentin, as determined by tetracycline staining and confocal microscopy. These cells also differentiated into vascular endothelial cells, as determined by beta-galactosidase staining of LacZ-tagged SHED. In vitro, vascular endothelial growth factor (VEGF) induced SHED to express VEGFR2, CD31, and VE-Cadherin (markers of endothelium) and to organize into capillary-like sprouts. VEGF induced ERK and AKT phosphorylation (indicative of differentiation), while inhibiting phosphorylation of STAT3 (indicative of 'stemness'). Collectively, this work demonstrates that SHED can differentiate into angiogenic endothelial cells and odontoblasts capable of generating tubular dentin.
引用
收藏
页码:791 / 796
页数:6
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