Survival of the probiotic, L-plantarum 299v and its effects on the faecal bacterial flora, with and without gastric acid inhibition

被引:42
作者
Goossens, D
Jonkers, D
Russel, M
Thijs, A
van den Bogaard, A
Stobberingh, E
Stockbrügger, R
机构
[1] State Univ Limburg Hosp, Dept Gastroenterol & Hepatol, NL-6202 AZ Maastricht, Netherlands
[2] State Univ Limburg Hosp, Dept Med Microbiol, NL-6202 AZ Maastricht, Netherlands
关键词
gastric acid inhibition; L; plantarum; 299v; probiotics;
D O I
10.1016/j.dld.2004.07.018
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Introduction. Probiotic bacteria have to survive passage through the gastrointestinal tract. In this placebo-controlled double-blind study, the effect of Lactobacillus plantarum 299v on the faecal flora was studied with and without gastric acid inhibition. Methods. Thirty-two healthy volunteers were given pantoprazole (40mg/day) or placebo for 3 weeks from week 2 until week 4. In addition, from week 3 until week 4, L. plantarum 299v in an oatmeal-fermented drink (10(9)CFU/ml) was given twice daily to both groups. From each healthy volunteer, faecal samples were collected at the end of week 1, 2, 4 and 8 (4 weeks after cessation of L. plantarum 299v and pantoprazole/placebo). Several aerobically and anaerobically growing bacteria were counted and short chain fatty acid concentrations were determined. Results. In both the pantoprazole and the placebo group, median lactobacilli counts increased significantly in week 4 compared to week I (from log 4.5 to 8.0 CFU/g faeces in pantoprazole and from log 4.2 to 7.7 CFU/g facces in placebo group) and decreased significantly in week 8 (to log 4.5 CFU/g faeces in pantoprazole and log 4.3 CFU/g faeces in placebo group). These lactobacilli were identified as L. plantarum 299v. No significant differences were observed in all other bacterial counts and short chain fatty acid concentrations. Conclusions. The comparable increase of faecal lactobacilli counts in both the pantoprazole and the placebo-treated group demonstrates that L. plantarum 299v survives passage through the gastrointestinal tract irrespective of gastric acidity. The increment of the intra-gastric pH in combination with L. plantarum 299v did not modulate bacterial composition and/or the production of short chain fatty acids. (C) 2004 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
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页码:44 / 50
页数:7
相关论文
共 31 条
[1]   SHORT-CHAIN FATTY-ACID CONTENT AND PH IN CECUM OF RATS GIVEN VARIOUS SOURCES OF CARBOHYDRATES [J].
BERGGREN, AM ;
BJORCK, IME ;
NYMAN, EMGL ;
EGGUM, BO .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1993, 63 (04) :397-406
[2]   Pantoprazole - An update of its pharmacological properties and therapeutic use in the management of acid-related disorders [J].
Cheer, SM ;
Prakash, A ;
Faulds, D ;
Lamb, HM .
DRUGS, 2003, 63 (01) :101-132
[3]   Meta-analysis: the effect of probiotic administration on antibiotic-associated diarrhoea [J].
Cremonini, F ;
Di Caro, S ;
Nista, EC ;
Bartolozzi, F ;
Capelli, G ;
Gasbarrini, G ;
Gasbarrini, A .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2002, 16 (08) :1461-1467
[4]  
Drouault S, 1999, APPL ENVIRON MICROB, V65, P4881
[5]   Favourable effect of an acidified milk (LC-1) on Helicobacter pylori gastritis in man [J].
Felley, CP ;
Corthésy-Theulaz, I ;
Rivero, JLB ;
Sipponen, P ;
Kaufmann, M ;
Bauerfeind, P ;
Wiesel, PH ;
Brassart, D ;
Pfeifer, A ;
Blum, AL ;
Michetti, P .
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 2001, 13 (01) :25-29
[6]   GASTRIC-ACID BARRIER TO INGESTED MICROORGANISMS IN MAN - STUDIES IN-VIVO AND IN-VITRO [J].
GIANNELLA, RA ;
ZAMCHECK, N ;
BROITMAN, SA .
GUT, 1972, 13 (04) :251-+
[7]   Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: A double-blind, placebo-controlled trial [J].
Gionchetti, P ;
Rizzello, F ;
Venturi, A ;
Brigidi, P ;
Matteuzzi, D ;
Bazzocchi, G ;
Poggioli, G ;
Miglioli, M ;
Campieri, M .
GASTROENTEROLOGY, 2000, 119 (02) :305-309
[8]   EFFECT OF DIET AND LACTOBACILLUS-ACIDOPHILUS SUPPLEMENTS ON HUMAN FECAL BACTERIAL ENZYMES [J].
GOLDIN, BR ;
SWENSON, L ;
DWYER, J ;
SEXTON, M ;
GORBACH, SL .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1980, 64 (02) :255-261
[9]   SURVIVAL OF LACTOBACILLUS SPECIES (STRAIN GG) IN HUMAN GASTROINTESTINAL-TRACT [J].
GOLDIN, BR ;
GORBACH, SL ;
SAXELIN, M ;
BARAKAT, S ;
GUALTIERI, L ;
SALMINEN, S .
DIGESTIVE DISEASES AND SCIENCES, 1992, 37 (01) :121-128
[10]   The effect of Lactobacillus plantarum 299v on the bacterial composition and metabolic activity in faeces of healthy volunteers:: a placebo-controlled study on the onset and duration of effects [J].
Goossens, D ;
Jonkers, D ;
Russel, M ;
Stobberingh, E ;
van den Bogaard, A ;
Stockbrügger, R .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2003, 18 (05) :495-505