Characterization of gentarnicin-resistant Mycoplasma hyorhinis

被引：3

作者：

Idowu, AD ^{[1
]}

Celones, MC ^{[1
]}

Salgado, AE ^{[1
]}

Marquez, KM ^{[1
]}

Pleibel, N ^{[1
]}

机构：

[1] Genentech Inc, Qual Control Biol, San Francisco, CA 94080 USA

来源：

BIOLOGICALS | 2003年 / 31卷 / 03期

关键词：

Mycoplasma hyorhinis; gentamicin resistance; mycoplasma detection; characterization; validation;

D O I：

10.1016/S1045-1056(03)00036-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A study was conducted to determine if a gentamicin-resistant strain of mycoplasma could be developed for use in validating current mycoplasma detection methods for biologic product harvest cell culture fluid (CCF) containing gentamicin. A strain of gentamicin-resistant Mycoplasma hyorhinis was isolated and characterized. The study showed that this organism was similar to the wild-type strain in all ways examined except gentamicin resistance. Both strains of mycoplasma (the gentamicin resistant and the wild-type) exhibited comparable growth patterns and showed 100% homology based on DNA sequencing and analysis of a 464-bp PCR product. Also, analysis using species-specific antisera identified both strains as M. hyorhinis. Two commonly used lot release mycoplasma detection methods (culture and DNAF) consistently detected mycoplasmas in spiked biologic product harvest CCF containing gentamicin but not in unspiked samples. This study demonstrates the first isolation and characterization of a gentamicin-resistant M. hyorhinis that can be used to validate mycoplasma detection methods for biologic product harvest CCF containing gentamicin. (C) 2003 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

引用

页码：175 / 179

页数：5

共 15 条

[1] IDENTIFICATION AND SOURCES OF MYCOPLASMAS ISOLATED FROM CONTAMINATED CELL-CULTURES [J].

BARILE, MF ;

HOPPS, HE ;

GRABOWSK.MW ;

RIGGS, DB ;

DELGIUDI.RA .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1973, 225 (DEC14) :251-264

[2]

BENTON WJ, 1967, AVIAN DIS, V3, P426

[3] SUSCEPTIBILITY OF GENITAL MYCOPLASMAS TO ANTIMICROBIAL AGENTS [J].

BRAUN, P ;

KLEIN, JO ;

KASS, EH .

APPLIED MICROBIOLOGY, 1970, 19 (01) :62-+

[4]

*EUR PHARM, 2001, MYCOPLASMAS S

[5]

FDA, 1993, POINTS CONS CHAR CEL

[6]

GENARO AR, 1985, REMINGTONS PHARM SCI, P1180

[7]

HAYFLICK L, 1965, TEX REP BIOL MED, VS 23, P285

[8] HUMAN RESPIRATORY-TRACT INFECTIONS WITH MYCOPLASMAS AND THEIR INVITRO SUSCEPTIBILITY TO TETRACYCLINES AND SOME OTHER ANTIBIOTICS [J].

MARDH, PA .

CHEMOTHERAPY, 1975, 21 :47-57

[9]

MASOVER G, 1981, PROKARYOTES, P2247

[10]

MASOVER GK, 1996, AUTOMATED MICROBIAL, P149

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