Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer

Proliferating cell nuclear antigen (PCNA) is involved in a wide range of functions in the nucleus. However, a substantial amount of PCNA is also present in the cytoplasm, although their function is unknown. Here we show, through Far-Western blotting and mass spectrometry, that PCNA is associated with several cytoplasmic oncoproteins, including elongation factor, malate dehydrogenase, and peptidyl-prolyl isomerase. Surprisingly, PCNA is also associated with six glycolytic enzymes that are involved in the regulation of steps 4-9 in the glycolysis pathway. Structured summary: MINT-7995351: G3P (uniprotkb:P04406) and PCNA (uniprotkb: P12004) colocalize (MI:0403) by fluorescence microscopy (MI: 0416) MINT-7995334: ENOA (uniprotkb:P06733) and PCNA (uniprotkb: P12004) colocalize (MI: 0403) by fluorescence microscopy (MI: 0416) MINT- 7995368: ALDOA (uniprotkb:P04075) and PCNA (uniprotkb: P12004) colocalize (MI: 0403) by fluorescence microscopy (MI: 0416) MINT- 7995141: G3P (uniprotkb: P04406) binds (MI: 0407) to PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995182: ENOA (uniprotkb: P06733) binds (MI: 0407) to PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995132: G3P (uniprotkb: P04406) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995228: PRDX6 (uniprotkb:P30041) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995220: CAH2 (uniprotkb:P00918) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995114: Triosephosphate isomerase (uniprotkb:P60174) binds (MI: 0407) to PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995244: K2C7 (uniprotkb:P08729) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995252: ANXA2 (uniprotkb:P07355) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995122: Triosephosphate isomerase (uniprotkb: P60174) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995093: ALDOA (uniprotkb: P04075) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT- 7995148: PGK1 (uniprotkb:P00558) physically interacts (MI: 0915) with PCNA (uniprotkb: P12004) by far western blotting (MI: 0047) MINT-7995158: PGAM1 (uniprotkb:P18669) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995166: PGAM1 (uniprotkb:P18669) binds (MI:0407) to PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995105: ALDOA (uniprotkb:P04075) binds (MI:0407) to PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995260: PPIA (uniprotkb:P62937) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995173: ENOA (uniprotkb:P06733) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995268: EF1A (uniprotkb:P68104) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995236: MDHM (uniprotkb:P40926) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995189: RSSA (uniprotkb:P08865) physically interacts (MI:0915) with PCNA (uniprotkb:P12004) by far western blotting (MI:0047) MINT-7995282: PCNA (uniprotkb:P12004) physically interacts (MI:0915) with ALDOA (uniprotkb:P00883) and G3P (uniprotkb:P46406) by anti bait coimmunoprecipitation (MI:0006). (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.