Anandamide synthesis is induced by arachidonate mobilizing agonists in cells of the immune system

被引:87
作者
Pestonjamasp, VK [1 ]
Burstein, SH [1 ]
机构
[1] Univ Massachusetts, Sch Med, Dept Biochem & Mol Biol, Worcester, MA 01655 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1998年 / 1394卷 / 2-3期
关键词
anandamide; cannabinoid; platelet-activating factor; lipopolysaccharide; arachidonic acid; macrophage;
D O I
10.1016/S0005-2760(98)00110-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hypothesis that the capability of agents to mobilize arachidonic acid (AA) could predict increased anandamide (ANA) synthesis in a macrophage cell line has been examined. Lipopolysaccharide (LPS), platelet-activating factor (PAF) and cannabinoids such as Delta(9)-tetrahydrocannabinol (THC) and anandamide were all found to be agonists for the release of AA and led to increased ANA synthesis in RAW264.7 mouse macrophage cells. Nitric oxide, in contrast, stimulated AA release without raising ANA levels. ANA stimulation of its own synthesis indicates the existence of a positive feedback mechanism. The possible involvement of the CB2 receptor in THC-mediated AA release and ANA synthesis is addressed using the antagonist SR144528. ANA synthesis is also increased by the combination of calcium ionophore and indomethacin, suggesting that ANA is metabolized by a cyclooxygenase in this system. The data imply that ANA could play a role in the response of the immune system to cannabinoids and bacterial endotoxins and that AA mobilization is a predictor for increased ANA synthesis. (C) 1998 Published by Elsevier Science B.V. AU rights reserved.
引用
收藏
页码:249 / 260
页数:12
相关论文
共 36 条
[1]  
BACHUR NR, 1966, J BIOL CHEM, V241, P1308
[2]   Biosynthesis, uptake, and degradation of anandamide and palmitoylethanolamide in leukocytes [J].
Bisogno, T ;
Maurelli, S ;
Melck, D ;
DePetrocellis, L ;
DiMarzo, V .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (06) :3315-3323
[3]   PHOSPHOLIPASE PARTICIPATION IN CANNABINOID-INDUCED RELEASE OF FREE ARACHIDONIC-ACID [J].
BURSTEIN, S ;
BUDROW, J ;
DEBATIS, M ;
HUNTER, SA ;
SUBRAMANIAN, A .
BIOCHEMICAL PHARMACOLOGY, 1994, 48 (06) :1253-1264
[4]  
BURSTEIN S, 1992, MARIJUANA CANNABINOI, P73
[5]   STIMULATION OF ANANDAMIDE BIOSYNTHESIS IN N-18TG2 NEUROBLASTOMA-CELLS BY DELTA(9)-TETRAHYDROCANNABINOL (THC) [J].
BURSTEIN, SH ;
HUNTER, SA .
BIOCHEMICAL PHARMACOLOGY, 1995, 49 (06) :855-858
[6]  
Cadas H, 1996, J NEUROSCI, V16, P3934
[7]   PLATELET-ACTIVATING-FACTOR - RECEPTORS AND SIGNAL-TRANSDUCTION [J].
CHAO, W ;
OLSON, MS .
BIOCHEMICAL JOURNAL, 1993, 292 :617-629
[8]   ALTERED RESPONSES TO MODULATORS OF GUANINE-NUCLEOTIDE BINDING-PROTEIN ACTIVITY IN ENDOTOXIN TOLERANCE [J].
COFFEE, KA ;
HALUSHKA, PV ;
WISE, WC ;
COOK, JA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1035 (02) :201-205
[9]   ENZYMATIC-SYNTHESIS AND DEGRADATION OF ANANDAMIDE, A CANNABINOID RECEPTOR AGONIST [J].
DEUTSCH, DG ;
CHIN, SA .
BIOCHEMICAL PHARMACOLOGY, 1993, 46 (05) :791-796
[10]   ENZYMATIC-SYNTHESIS OF ANANDAMIDE, AN ENDOGENOUS LIGAND FOR THE CANNABINOID RECEPTOR, BY BRAIN MEMBRANES [J].
DEVANE, WA ;
AXELROD, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6698-6701