Induction of cell-cell detachment during glucose starvation through F-actin conversion by SNARK, the fourth member of the AMP-activated protein kinase catalytic subunit family

被引：35

作者：

Suzuki, A ^{[1
]}

Kusakai, G ^{[1
]}

Kishimoto, A ^{[1
]}

Minegichi, Y ^{[1
]}

Ogura, T ^{[1
]}

Esumi, H ^{[1
]}

机构：

[1] Natl Canc Ctr, Res Inst E, Investigat Treatment Div, Kashiwa, Chiba 2778577, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 311卷 / 01期

关键词：

protein kinase; SNARK; AMPK; glucose starvation; cell-cell detachment;

D O I：

10.1016/j.bbrc.2003.09.184

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SNARK, the fourth member of the AMPK catalytic subunit family, was originally identified in a rat kidney cDNA library, and in this study we isolated its human homologue. A BLAST search analysis using rat SNARK protein yielded a single high homology clone, DKFZp434J037, isolated from human testis, and since its hypothetical protein showed 84% homology to rat SNARK protein, we assumed DKFZp434J037 to be the human SNARK cDNA. The human SNARK cDNA is 3443 bp long and encodes a 628 amino acid protein having an estimated molecular weight of 69 kDa, and its chromosomal localization had been assigned to 1q32.1. The same as other members of AMPK catalytic subunit family, human SNARK showed AMP-dependent GST-SAMS phosphorylation activity and enhanced HepG2 cell survival during glucose, starvation. Human SNARK-overexpressing HepG2 cells (H/SNK) showed acute cell-cell detachment when exposed to glucose-free medium and the cell-cell detachment correlated well with the detection of G-actin. Deletion mutant analysis strongly suggested that the putative catalytic domain of SNARK is necessary for the cell-cell detachment, and Western blotting analysis showed that phosphorylation of FAK and PKC, which were dramatically increased by glucose starvation in HepG2 cells, was markedly suppressed by SNARK. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：156 / 161

页数：6

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