Structural organization and chromosomal location of the chicken α-amylase gene family

被引:7
作者
Benkel, BF
Nguyen, T
Uno, Y
de León, FAP
Hickey, DA
机构
[1] Agr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada
[2] Agr & Agri Food Canada, Ctr Food & Anim Res, Ottawa, ON K1A 0C6, Canada
[3] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA
[4] Concordia Univ, Dept Biol, Montreal, PQ H4B 1R6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
gene duplication; pseudogenes; fluorescent in situ hybridzation;
D O I
10.1016/j.gene.2005.07.030
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Characterization of the Gallus gallus alpha-amylase gene family revealed that the chicken genome contains two distinct amy loci. One of the two loci is expressed in the chicken pancreas while cDNA clones for the second locus were detected in a library Constructed from liver mRNA. Fluorescent in situ hybridization to chromosome spreads showed that the two loci are both located on chromosome 8 within the chicken genome. Moreover, each locus contains both in intact, expressed gene copy as well as a pseudogene. The expressed gene and the pseudogene are arranged in a divergent configuration in the pancreatic amy locus, while in the hepatic locus the intact gene and the pseudogene are arranged in tandem. The data suggest a complex pattern of evolution for the chicken amylase gene family which includes multiple gene duplication events, insertion/deletion events, as well as changes in spatial expression patterns. Crown Copyright (c) 2005 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:117 / 124
页数:8
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