Lytic activity of recombinant bacteriophage φ11 and φ12 endolysins on whole cells and biofilms of Staphylococcus aureus

被引：170

作者：

Sass, Peter ^{[1
]}

Bierbaum, Gabriele ^{[1
]}

机构：

[1] Univ Bonn, IMMIP, D-53105 Bonn, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 01期

关键词：

D O I：

10.1128/AEM.01616-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The recombinant phi 11 endolysin hydrolyzed heat-killed staphylococci as well as staphylococcal biofilms. Cell wall targeting appeared to be a prerequisite for lysis of whole cells, and the combined action of the endopeptidase and amidase domains was necessary for maximum activity. In contrast, the phi 12 endolysin was inactive and caused aggregation of the cells.

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页码：347 / 352

页数：6

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