Transient repressor effect of Fis on the growth phase-regulated osmE promoter of Escherichia coli K12

被引:9
作者
Bordes, P
Bouvier, J
Conter, A
Kolb, A
Gutierrez, C
机构
[1] Univ Toulouse 3, CNRS, UMR 5100, Lab Microbiol & Genet Mol, F-31062 Toulouse, France
[2] Inst Pasteur, Lab Regulat Transciptionnelles, F-75724 Paris 15, France
关键词
Escherichia coli; transcriptional regulation; Fis; stationary phase; osmE;
D O I
10.1007/s00438-002-0733-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription of the gene osmE of Escherichia coli is osmotically inducible and regulated by the growth phase. Expression of osmE is directed by a single promoter, osmE(p), which is recognized by Esigma(70) and Esigma(s), two forms of RNA polymerase using, respectively, the sigma factors sigma(70) and sigma(s). Esigma(s) transcribes osmEp during entry into stationary phase. Esigma(70) is responsible for osmotic induction of osmEp during the exponential growth phase. In a search for proteins that can modulate osmE. expression in trans, we performed electrophoretic mobility shift experiments using a DNA fragment carrying osmEp and crude extracts from E. coli. One major retarded band was observed in these experiments. The Fis protein is responsible for this retarded band, and binds to several sites upstream and downstream of, and overlapping, the promoter region of osmE. In a fis mutant background, the kinetics of in vivo transcription of osmEp during growth demonstrated that Fis is not responsible for the repression of the promoter seen during early exponential phase. In contrast, expression of osmE(p) at elevated osmolarity during the mid-exponential growth phase is increased in the absence of Fis, demonstrating that Fis is able to act as a repressor in vivo at a particular stage of growth.
引用
收藏
页码:206 / 213
页数:8
相关论文
共 36 条
[1]   Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid [J].
Azam, TA ;
Iwata, A ;
Nishimura, A ;
Ueda, S ;
Ishihama, A .
JOURNAL OF BACTERIOLOGY, 1999, 181 (20) :6361-6370
[2]   DRAMATIC CHANGES IN FIS LEVELS UPON NUTRIENT UPSHIFT IN ESCHERICHIA-COLI [J].
BALL, CA ;
OSUNA, R ;
FERGUSON, KC ;
JOHNSON, RC .
JOURNAL OF BACTERIOLOGY, 1992, 174 (24) :8043-8056
[3]   INTERACTION OF FIS PROTEIN WITH DNA - BENDING AND SPECIFICITY OF BINDING [J].
BETERMIER, M ;
GALAS, DJ ;
CHANDLER, M .
BIOCHIMIE, 1994, 76 (10-11) :958-967
[4]  
BETERMIER M, 1993, J BACTERIOL, V175, P3798
[5]   Involvement of differential efficiency of transcription by Eσs and Eσ70 RNA polymerase holoenzymes in growth phase regulation of the Escherichia coli osmE promoter [J].
Bordes, P ;
Repoila, F ;
Kolb, A ;
Gutierrez, C .
MOLECULAR MICROBIOLOGY, 2000, 35 (04) :845-853
[6]   Interplay between global regulators of Escherichia coli:: effect of RpoS, Lrp and H-NS on transcription of the gene osmC [J].
Bouvier, J ;
Gordia, S ;
Kampmann, G ;
Lange, R ;
Hengge-Aronis, R ;
Gutierrez, C .
MOLECULAR MICROBIOLOGY, 1998, 28 (05) :971-980
[7]   Suppression of FNR-dependent transcription activation at the Escherichia coli nir promoter by Fis, IHF and H-NS:: modulation of transcription initiation by a complex nucleo-protein assembly [J].
Browning, DF ;
Cole, JA ;
Busby, SJW .
MOLECULAR MICROBIOLOGY, 2000, 37 (05) :1258-1269
[8]   TRANSPOSITION AND FUSION OF LAC GENES TO SELECTED PROMOTERS IN ESCHERICHIA-COLI USING BACTERIOPHAGE-LAMBDA AND BACTERIOPHAGE-MU [J].
CASADABAN, MJ .
JOURNAL OF MOLECULAR BIOLOGY, 1976, 104 (03) :541-555
[9]   σ factor selectivity of Escherichia coli RNA polymerase:: role for CRP, IHF and Lrp transcription factors [J].
Colland, F ;
Barth, M ;
Hengge-Aronis, R ;
Kolb, A .
EMBO JOURNAL, 2000, 19 (12) :3028-3037
[10]   Role of DNA supercoiling and RpoS sigma factor in the osmotic and growth phase-dependent induction of the gene osmE of Escherichia coli K12 [J].
Conter, A ;
Menchon, C ;
Gutierrez, C .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (01) :75-83