Development of a disposable biosensor for the detection of metam-sodium and its metabolite MITC

被引：26

作者：

Noguer, T

Balasoiu, AM

Avramescu, A

Marty, JL

机构：

[1] Univ Perpignan, UMR CNRS 5054, Ctr Phytopharm, F-66860 Perpignan, France

[2] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest 76234, Romania

来源：

ANALYTICAL LETTERS | 2001年 / 34卷 / 04期

关键词：

screen-printing; disposable electrode; metam-sodium; MITC; aldehyde dehydrogenase; Meldola's Blue; PVA SbQ entrapment;

D O I：

10.1081/AL-100002592

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A disposable monoenzymatic sensor for the reliable determination of dithiocarbamate fungicides was developed based on aldehyde dehydrogenase inhibition. Aldehyde dehydrogenase was immobilized on the surface of a disposable screen-printed carbon-paste electrode by using a photocross-linkable poly-(vinylalcohol) bearing styrylpyridinium groups. Electrochemical oxidation of NADH was carried out at a potential as low as 0V vs Ag/AgCl using Meldola's Blue as mediator. The sensor was used for the determination of metam-sodium, a soil fumigant, and its main metabolite MITC. It was shown that metam-sodium did not inhibit AIDH while MITC could be detected at levels of 100 ppb. The sensor thus allowed to discrimate between metam-sodium and its toxic metabolite MITC.

引用

收藏

页码：513 / 528

页数：16

相关论文

共 29 条

[1] Development of an amperometric assay for NH4+_ based on a chemically modified screen-printed NADH sensor [J].

Abass, AK ;

Hart, JP ;

Cowell, DC ;

Chappell, A .

ANALYTICA CHIMICA ACTA, 1998, 373 (01) :1-8

[2] Amperometric glutamate biosensor based on poly(o-phenylenediamine) film electrogenerated onto modified carbon paste electrodes [J].

Alvarez-Crespo, SL ;

Lobo-Castanon, MJ ;

Miranda-Ordieres, AJ ;

Tunon-Blanco, P .

BIOSENSORS & BIOELECTRONICS, 1997, 12 (08) :739-747

[3] AMPEROMETRIC ASSAY FOR THE KETONE-BODY 3-HYDROXYBUTYRATE [J].

BATCHELOR, MJ ;

GREEN, MJ ;

SKETCH, CL .

ANALYTICA CHIMICA ACTA, 1989, 221 (02) :289-294

[4] AN AMPEROMETRIC GLUCOSE ELECTRODE BASED ON CARBON PASTE, CHEMICALLY MODIFIED WITH GLUCOSE-DEHYDROGENASE, NICOTINAMIDE ADENINE-DINUCLEOTIDE, AND A PHENOXAZINE MEDIATOR, COATED WITH A POLY(ESTER SULFONIC-ACID) CATION EXCHANGER [J].

BREMLE, G ;

PERSSON, B ;

GORTON, L .

ELECTROANALYSIS, 1991, 3 (02) :77-86

[5] ANALYSIS OF METHYLISOTHIOCYANATE DERIVED FROM THE SOIL FUMIGANT METHAM-SODIUM IN WORKROOM AIR [J].

COLLINA, A ;

MAINI, P .

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1979, 22 (03) :400-404

[6] Alternative fumigants for methyl bromide in tobacco and pepper transplant production [J].

Csinos, AS ;

Johnson, WC ;

Johnson, AW ;

Sumner, DR ;

McPherson, RM ;

Gitaitis, RD .

CROP PROTECTION, 1997, 16 (06) :585-594

[7] Methyl bromide alternatives in tobacco, tomato and pepper transplant production [J].

Csinos, AS ;

Sumner, DR ;

Johnson, WC ;

Johnson, AW ;

McPherson, RM ;

Dowler, CC .

CROP PROTECTION, 2000, 19 (01) :39-49

[8] REAGENTLESS CHEMICALLY-MODIFIED CARBON-PASTE ELECTRODE BASED ON A PHENOTHIAZINE POLYMER DERIVATIVE AND YEAST ALCOHOL-DEHYDROGENASE FOR THE ANALYSIS OF ETHANOL [J].

DOMINGUEZ, E ;

LAN, HL ;

OKAMOTO, Y ;

HALE, PD ;

SKOTHEIM, TA ;

GORTON, L ;

HAHNHAGERDAL, B .

BIOSENSORS & BIOELECTRONICS, 1993, 8 (3-4) :229-237

[9] CHEMICALLY MODIFIED ELECTRODES FOR THE ELECTROCATALYTIC OXIDATION OF NICOTINAMIDE COENZYMES [J].

GORTON, L .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1986, 82 :1245-1258

[10] SELECTIVE DETECTION IN FLOW-ANALYSIS BASED ON THE COMBINATION OF IMMOBILIZED ENZYMES AND CHEMICALLY MODIFIED ELECTRODES [J].

GORTON, L ;

CSOREGI, E ;

DOMINGUEZ, E ;

EMNEUS, J ;

JONSSONPETTERSSON, G ;

MARKOVARGA, G ;

PERSSON, B .

ANALYTICA CHIMICA ACTA, 1991, 250 (01) :203-248