Agmatine treatment and vein graft reconstruction enhance recovery after experimental facial nerve injury

被引:20
作者
Berenholz, L
Segal, S
Gilad, VH
Klein, C
Yehezkeli, E
Eviatar, E
Kessler, A
Gilad, GM
机构
[1] Tel Aviv Univ, Dept Physiol & Pharmacol, Sackler Fac Med, IL-67639 Tel Aviv, Israel
[2] Tel Aviv Univ, Dept Neurol, Sackler Fac Med, Assaf Harofeh Med Ctr, IL-67639 Tel Aviv, Israel
[3] Tel Aviv Univ, Dept Otolaryngol, Sackler Fac Med, Assaf Harofeh Med Ctr, IL-67639 Tel Aviv, Israel
[4] Wolfson Govt Hosp, Dept Otolaryngol, Holon, Israel
关键词
agmatine; facial nerve reconstruction; nerve regeneration; vein graft; vibrissae movements;
D O I
10.1111/j.1085-9489.2005.10310.x
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
The rate of nerve regeneration is a critical determinant of the degree of functional recovery after injury. Here, we sought to determine whether treatment with the neuroprotective compound, agmatine, with or without nerve reconstruction utilizing a regional autogenous vein graft would accelerate the rate of facial nerve regeneration. Experiments compared the following seven groups of adult male rats: (A) Intact untreated controls. (B) Sham operation with interruption of the nerve blood supply (controls). (C) Transection of the mandibular branch of the facial nerve (generating a gap of 3 mm) followed by saline treatment. (D) Nerve transection with unsutured autogenous vein (external jugular) graft reconstruction plus saline treatment. (E) Nerve transection with sutured vein graft approximation (coaptation of the proximal and distal nerve stumps) plus saline. (F) Nerve transection with sutured vein graft followed by agmatine treatment (four daily intraperitoneal injections of 100 mg/kg agmatine sulfate). (G) Nerve transection with unsutured vein graft followed by agmatine treatment. Functional recovery, as assessed by grading vibrissae movements and by recording nerve conduction velocity and numbers of regenerated axons, indicated that either vein reconstruction or agmatine treatment resulted in accelerated and more complete recovery as compared with controls. But best results were observed in animals that underwent combined treatment, i.e., vein reconstruction plus agmatine injection. We conclude that agmatine treatment can accelerate facial nerve regeneration and that agmatine treatment together with autogenous vein graft offers an advantageous alternative to other facial nerve reconstruction procedures.
引用
收藏
页码:319 / 328
页数:10
相关论文
共 72 条
[1]   SELECTIVE REINNERVATION OF SOMATOTOPICALLY APPROPRIATE MUSCLES AFTER FACIAL-NERVE TRANSECTION AND REGENERATION IN THE NEONATAL RAT [J].
ALDSKOGIUS, H ;
THOMANDER, L .
BRAIN RESEARCH, 1986, 375 (01) :126-134
[2]   SELECTIVE-INHIBITION OF INDUCIBLE NITRIC-OXIDE SYNTHASE BY AGMATINE [J].
AUGUET, M ;
VIOSSAT, I ;
MARIN, JG ;
CHABRIER, PE .
JAPANESE JOURNAL OF PHARMACOLOGY, 1995, 69 (03) :285-287
[3]  
BERENHOLZ L, 1998, 2 CRAN FAC INT S JER
[4]   Prevention by lamotrigine, MK-801 and N-omega-nitro-L-arginine methyl ester of motoneuron cell death after neonatal axotomy [J].
Casanovas, A ;
Ribera, J ;
Hukkanen, M ;
RiverosMoreno, V ;
Esquerda, JE .
NEUROSCIENCE, 1996, 71 (02) :313-325
[5]   EARLY POLYAMINE TREATMENT ACCELERATES REGENERATION OF RAT SYMPATHETIC NEURONS [J].
DORNAY, M ;
GILAD, VH ;
SHILER, I ;
GILAD, GM .
EXPERIMENTAL NEUROLOGY, 1986, 92 (03) :665-674
[6]   Agmatine reverses pain induced by inflammation, neuropathy, and spinal cord injury [J].
Fairbanks, CA ;
Schreiber, KL ;
Brewer, KL ;
Yu, CG ;
Stone, LS ;
Kitto, KF ;
Nguyen, HO ;
Grocholski, BM ;
Shoeman, DW ;
Kehl, LJ ;
Regunathan, S ;
Reis, DJ ;
Yezierski, RP ;
Wilcox, GL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (19) :10584-10589
[7]  
FU SY, 1995, J NEUROSCI, V15, P3886
[8]   INCREASED NUMBER OF SYMPATHETIC NEURONS WITH UNCHANGED TARGET ORGAN INNERVATION AFTER POSTNATAL POLYAMINE TREATMENT [J].
GILAD, GM ;
DORNAY, M ;
GILAD, VH .
DEVELOPMENTAL BRAIN RESEARCH, 1986, 28 (02) :163-168
[9]  
GILAD GM, 1983, EXP NEUROL, V81, P158, DOI 10.1016/0014-4886(83)90165-6
[10]   POLYAMINE BIOSYNTHESIS IS REQUIRED FOR SURVIVAL OF SYMPATHETIC NEURONS AFTER AXONAL INJURY [J].
GILAD, GM ;
GILAD, VH .
BRAIN RESEARCH, 1983, 273 (01) :191-194