Antagonistic Activities of Lactobacilli against Helicobacter pylori Growth and Infection in Human Gastric Epithelial Cells

被引：60

作者：

Chen, Xiaohua ^{[1
]}

Liu, Xiao-ming ^{[2
]}

Tian, Fengwei

Zhang, Qiuxiang ^{[2
]}

Zhang, He-ping ^{[2
,3
]}

Zhang, Hao

Chen, Wei ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[3] Inner Mongolia Agr Univ, Key Lab Dairy Biotechnol & Engn, Minist Educ, Hohhot 010018, Inner Mongolia, Peoples R China

来源：

JOURNAL OF FOOD SCIENCE | 2012年 / 77卷 / 01期

关键词：

gastric epithelial cells; Helicobacter pylori; lactobacilli; LACTIC-ACID BACTERIA; IN-VITRO; JOHNSONII LA1; STRAIN; INHIBITION; SALIVARIUS; GG;

D O I：

10.1111/j.1750-3841.2011.02498.x

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Lactobacilli have positive effects on bowel microflora and health in humans and animals. In this study, the antagonistic activities of Lactobacillus gasseri Chen, and L. plantarum 18 were assessed by agar plate diffusion assay and tests that determined the growth and urease activity of Helicobacter pylori cocultured with lactobacilli and the adherence of H. pylori to human gastric epithelial cells in the presence of lactobacilli. The results showed that the 2 Lactobacillus strains had significant anti-H.pylori activity, and this activity may be contributed by the cell-free supernatants (CFS) of lactobacilli and live Lactobacillus strains in vitro. The antagonistic activity of the CFS against H. pylori depended on the pH and the presence of metabolites, such as organic acids and proteases. Our results also indicated that 2 Lactobacillus strains could inhibit H. pylori adherence human gastric epithelial cells.

引用

页码：M9 / M14

页数：6

共 30 条

[1]

Aiba Y, 1998, AM J GASTROENTEROL, V93, P2097, DOI 10.1111/j.1572-0241.1998.00600.x

[2]

The effect of oral administration of Lactobacillus GG on antibiotic-associated gastrointestinal side-effects during Helicobacter pylori eradication therapy [J].

Armuzzi, A ;

Cremonini, F ;

Bartolozzi, F ;

Canducci, F ;

Candelli, M ;

Ojetti, V ;

Cammarota, G ;

Anti, M ;

De Lorenzo, A ;

Pola, P ;

Gasbarrini, G ;

Gasbarrini, A .

ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2001, 15 (02) :163-169

[3]

Salivaricin P, one of a family of two-component antilisterial bacteriocins produced by intestinal isolates of Lactobacillus salivarius [J].

Barrett, Eoin ;

Hayes, Maria ;

O'Connor, Paula ;

Gardiner, Gillian ;

Fitzgerald, Gerald F. ;

Stanton, Catherine ;

Ross, R. Paul ;

Hill, Colin .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (11) :3719-3723

[4]

GroEL of Lactobacillus johnsonii La1 (NCC 533) is cell surface associated:: Potential role in interactions with the host and the gastric pathogen Helicobacter pylori [J].

Bergonzelli, GE ;

Granato, D ;

Pridmore, RD ;

Marvin-Guy, LF ;

Donnicola, D ;

Corthésy-Theulaz, IE .

INFECTION AND IMMUNITY, 2006, 74 (01) :425-434

[5]

LACTOBACILLUS-ACIDOPHILUS LA-1 BINDS TO CULTURED HUMAN INTESTINAL-CELL LINES AND INHIBITS CELL ATTACHMENT AND CELL INVASION BY ENTEROVIRULENT BACTERIA [J].

BERNET, MF ;

BRASSART, D ;

NEESER, JR ;

SERVIN, AL .

GUT, 1994, 35 (04) :483-489

[6]

In vitro screening of lactobacilli with antagonistic activity against Helicobacter pylori from traditionally fermented foods [J].

Chen, X. ;

Tian, F. ;

Liu, X. ;

Zhao, J. ;

Zhang, H. -P. ;

Zhang, H. ;

Chen, W. .

JOURNAL OF DAIRY SCIENCE, 2010, 93 (12) :5627-5634

[7]

Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB [J].

Coconnier, MH ;

Lievin, V ;

BernetCamard, MF ;

Hudault, S ;

Servin, AL .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (05) :1046-1052

[8]

Two stomach-originated lactobacillus strains improve Helicobacter pylori infected murine gastritis [J].

Cui, Yi ;

Wang, Chun-Lian ;

Liu, Xiao-Wei ;

Wang, Xue-Hong ;

Chen, Lin-Lin ;

Zhao, Xin ;

Fu, Nian ;

Lu, Fang-Gen .

WORLD JOURNAL OF GASTROENTEROLOGY, 2010, 16 (04) :445-452

[9]

Mode of action of acidocin D20079, a bacteriocin produced by the potential probiotic strain, Lactobacillus acidophilus DSM 20079 [J].

Deraz, Sahar F. ;

Nordberg Karlsson, Eva ;

Khalil, Ashraf A. ;

Mattiasson, Bo .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2007, 34 (05) :373-379

[10]

Helicobacter pylori [J].

Dunn, BE ;

Cohen, H ;

Blaser, MJ .

CLINICAL MICROBIOLOGY REVIEWS, 1997, 10 (04) :720-+

← 1 2 3 →