Monitoring of a pyrite-oxidising bacterial population using DNA single-strand conformation polymorphism and microscopic techniques

被引:22
作者
Battaglia-Brunet, F
Clarens, M
d'Hugues, P
Godon, JJ
Foucher, S
Morin, D
机构
[1] Bur Rech Geol & Minieres, Environm & Proc Div, Biotechnol Unit, F-45060 Orleans 2, France
[2] BIO ID, Pepiniere Enterprise EOLE, F-11100 Narbonne, France
[3] INRA, Lab Environm Biotechnol, F-11100 Narbonne, France
关键词
D O I
10.1007/s00253-002-1095-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The single-strand conformation polymorphism (SSCP) technique was used to study the evolution of a bacterial consortium during the batch oxidation of a cobaltiferous pyrite in two types of bio-reactor: a bubble column and a classical stirred tank. Sequencing 16S rDNA revealed the presence of three organisms affiliated to Leptospirillum ferrooxidans, Acidithiobacillus thiooxidans and Sulfobacillus thermosulfidooxidans, respectively. Attempts were made to determine the proportions of bacteria attached to solid particles or freely suspended in the medium using a combination of PCR-SSCP and a microscopic technique. Ac. thiooxidans-related bacteria were dominant in the liquid during the early phase of the batch, but were later supplanted by L. ferrooxidans-related bacteria. L. ferrooxidans-related organisms were always in the majority on the solids. The growth of S. thermosulfidooxidans-related bacteria seemed to be favoured by the bubble-column reactor.
引用
收藏
页码:206 / 211
页数:6
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