Imbalance of Th17/Tregs in rats with smoke inhalation-induced acute lung injury

被引：57

作者：

Zhang, Fan

Li, Mian-yang

Lan, Ya-ting

Wang, Cheng-bin ^{[1
]}

机构：

[1] Chinese Peoples Liberat Army Gen Hosp, Dept Clin Lab Med, Beijing 100853, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

REGULATORY T-CELLS; GROWTH-FACTOR-BETA; NEUTROPHIL RECRUITMENT; TGF-BETA; TH17; INTERLEUKIN-17; INFLAMMATION; EXPRESSION; DISEASE; IL-17;

D O I：

10.1038/srep21348

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

T helper (Th) 17 cells and CD4(+) CD25(+) regulatory T (Treg) cells are supposed to be critically involved in regulating autoimmune and inflammatory diseases. The aim of this study was to investigate the Th17/Treg pattern in rats with gunpowder smog-induced acute lung injury. Wistar rats were equally randomized to three groups: normal control group, ALI 6 h group (smoke inhalation for 6 h) and ALI 24 h group (smoke inhalation for 24 h). We observed changes in cell counting in bronchoalveolar lavage fluid (BALF), alveolar-capillary membrane permeability and lung tissue pathology. Moreover, rats in ALI 6 h and ALI 24 h group showed increased expression of Th17 cell and related cytokines (IL-17 A, IL-6, TGF-beta and IL-23). Meanwhile, Treg prevalence and related cytokines (IL-10, IL-2 and IL-35) were decreased. Consequently, the ratio of Th17/Treg was higher after smoke inhalation. Additionally, Th1 cell decreased while Th2 cell increased at 6 h and 24 h after smoke inhalation. In conclusion, Th17/Treg imbalance exists in rats with smoke inhalation-induced acute lung injury, suggesting its potential role in the pathogenesis of this disease.

引用

页数：14

共 55 条

[1] An increased alveolar CD4+CD25+Foxp3+T-regulatory cell ratio in acute respiratory distress syndrome is associated with increased 30-day mortality [J].