Anterograde tracing of human hippocampus in vitro - a neuroanatomical tract tracing technique for the analysis of local fiber tracts in human brain

被引:8
作者
Freiman, TM
Gimbel, K
Honegger, E
Volk, B
Zentner, J
Frotscher, M
Deller, T
机构
[1] Univ Frankfurt, Inst Clin Neuroanat, D-60590 Frankfurt, Germany
[2] Univ Clin Freiburg, Neurozentrum, Dept Neurosurg, D-79106 Freiburg, Germany
[3] Univ Freiburg, Inst Anat, D-79106 Freiburg, Germany
[4] Univ Clin Freiburg, Neurozentrum, Dept Neuropathol, D-79106 Freiburg, Germany
关键词
hippocampal connectivity; mossy fiber; temporal lobe epilepsy; neurobiotin; electron microscopy; sprouting;
D O I
10.1016/S0165-0270(02)00187-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Hippocampal slices were obtained from hippocampi of patients with temporal lobe epilepsy or from patients with mass lesions located in the temporal lobe. Hippocampal slices were kept alive in a slice chamber and the anterograde tracer neurobiotin was iontophoretically injected into the granule cell layer of the dentate gyrus. Single injections resulted in the labeling of small groups of granule cells. The axonal arbor of these cells could be partially reconstructed and single mossy fibers could be followed from the soma to the inner molecular layer of the sclerotic dentate gyrus. Electron microscopy revealed asymmetric mossy fiber synapses on spiny neurons in the inner molecular layer, presumably granule cells. These data demonstrate that in vitro anterograde tracing can be employed to study the local connectivity of the human brain at the light and electron microscopic level. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:95 / 103
页数:9
相关论文
共 41 条
  • [1] Amaral David G., 1995, P443
  • [2] SYNAPTIC REORGANIZATION BY MOSSY FIBERS IN HUMAN EPILEPTIC FASCIA-DENTATA
    BABB, TL
    KUPFER, WR
    PRETORIUS, JK
    CRANDALL, PH
    LEVESQUE, MF
    [J]. NEUROSCIENCE, 1991, 42 (02) : 351 - 363
  • [3] Properties of a delayed rectifier potassium current in dentate granule cells isolated from the hippocampus of patients with chronic temporal lobe epilepsy
    Beck, H
    Blumcke, I
    Kral, T
    Clusmann, H
    Schramm, J
    Wiestler, OD
    Heinemann, U
    Elger, CE
    [J]. EPILEPSIA, 1996, 37 (09) : 892 - 901
  • [4] BOLAM JP, 1992, EXPT NEUROANATOMY PR, P1
  • [5] TRACING OF AXONAL CONNECTIONS BY RHODAMINE-DEXTRAN-AMINE IN THE RAT HIPPOCAMPAL-ENTORHINAL CORTEX SLICE PREPARATION
    BOULTON, CL
    VONHAEBLER, D
    HEINEMANN, U
    [J]. HIPPOCAMPUS, 1992, 2 (02) : 99 - 106
  • [6] CHEVASSUSAULOUI.N, 1997, EPILEPSY COMPREHENSI, P387
  • [7] Dai JP, 1998, J COMP NEUROL, V400, P87, DOI 10.1002/(SICI)1096-9861(19981012)400:1<87::AID-CNE6>3.0.CO
  • [8] 2-P
  • [9] Dai JP, 1998, J COMP NEUROL, V401, P16, DOI 10.1002/(SICI)1096-9861(19981109)401:1<16::AID-CNE2>3.0.CO
  • [10] 2-O