RCY1, an Arabidopsis thaliana RPP8/HRT family resistance gene, conferring resistance to cucumber mosaic virus requires salicylic acid, ethylene and a novel signal transduction mechanism

被引:181
作者
Takahashi, H
Miller, J
Nozaki, Y
Sukamto
Takeda, M
Shah, J
Hase, S
Ikegami, M
Ehara, Y
Dinesh-Kumar, SP
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Dept Life Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
[2] Yale Univ, Dept Mol Cellular & Dev Biol, OML 451, New Haven, CT 06520 USA
[3] Kansas State Univ, Div Biol, Mol Cellular & Dev Biol Program, Manhattan, KS 66506 USA
关键词
cucumber mosaic virus; virus resistance; CC-NBS-LRR protein; signal transduction; salicylic acid;
D O I
10.1046/j.1365-313X.2002.01453.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The dominant locus, RCY1, in the Arabidopsis thaliana ecotype C24 confers resistance to the yellow strain of cucumber mosaic virus ( CMV-Y). The RCY1 locus was mapped to a 150-kb region on chromosome 5. Sequence comparison of this region from C24 and a CMV-Y-susceptible C24 mutant predicts that the RCY1 gene encodes a 104-kDa CC-NBS-LRR-type protein. The RCY1 gene from C24, when expressed in the susceptible ecotype Wassilewskija (Ws), restricted the systemic spread of virus. RCY1 is allelic to the resistance genes RPP8 from the ecotype Landsberg erecta and HRT from the ecotype Dijon-17, which confer resistance to Peronospora parasitica biotype Emco5 and turnip crinkle virus (TCV), respectively. Examination of RCY1 plants defective in salicylic acid ( SA), jasmonic acid (JA) and ethylene signaling revealed a requirement for SA and ethylene signaling in mounting a resistance response to CMV-Y. The RCY1 nahG etr1 double mutants exhibited an intermediate level of susceptibility to CMV-Y, compared to the resistant ecotype C24 and the susceptible ecotypes Columbia and Nossen. This suggests that in addition to SA and ethylene, a novel signaling mechanism is associated with the induction of resistance in CMV-Y-infected C24 plants. Moreover, our results suggest that the signaling pathways downstream of the RPP8, HRT, and RCY1 have evolved independently.
引用
收藏
页码:655 / 667
页数:13
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