Snapshots of the reaction mechanism of matrix metalloproteinases

被引：88

作者：

Bertini, Ivano

Calderone, Vito

Fragai, Marco

Luchinat, Claudio

Maletta, Massimiliano

Yeo, Kwon Joo

机构：

[1] Univ Florence, CERM, I-50019 Sesto Fiorentino, Italy

[2] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

[3] Univ Florence, Dept Agr Biotechnol, I-50144 Florence, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 47期

关键词：

enzyme catalysis; metalloproteins; peptides reaction; mechanisms; X-ray diffraction;

D O I：

10.1002/anie.200603100

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) The series of events that occur in the catalytic cycle of matrix metalloproteinases were modeled on the basis of X-ray crystal structures of the active, uninhibited enzymes and of the same enzymes following the hydrolysis of a peptide substrate. After the peptide bond has been broken, both peptide fragments remain bound to the protein initially (see structure of the active-site cavity of the enzyme MMP-12 immediately after substrate hydrolysis). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：7952 / 7955

页数：4

相关论文

共 31 条

[1] Bioinformatic comparison of structures and homology-models of matrix metalloproteinases [J].

Andreini, C ;

Banci, L ;

Bertini, I ;

Luchinat, C ;

Rosato, A .

JOURNAL OF PROTEOME RESEARCH, 2004, 3 (01) :21-31

[2] X-ray structures of binary and ternary enzyme-product-Inhibitor complexes of matrix metalloproteinases [J].

Bertini, I ;

Calderone, V ;

Fragai, M ;

Luchinat, C ;

Mangani, S ;

Terni, B .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (23) :2673-2676

[3] P-KAPPA-A OF ZINC-BOUND WATER AND NUCLEOPHILICITY OF HYDROXO-CONTAINING SPECIES - ABINITIO CALCULATIONS ON MODELS FOR ZINC ENZYMES [J].

BERTINI, I ;

LUCHINAT, C ;

ROSI, M ;

SGAMELLOTTI, A ;

TARANTELLI, F .

INORGANIC CHEMISTRY, 1990, 29 (08) :1460-1463

[4] Conformational variability of matrix metalloproteinases: Beyond a single 3D structure [J].

Bertini, I ;

Calderone, V ;

Cosenza, M ;

Fragai, M ;

Lee, YM ;

Luchinat, C ;

Mangani, S ;

Terni, B ;

Turano, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (15) :5334-5339

[5]

Bertini I, 2003, ANGEW CHEM, V115, P2777

[6] PAR1 is a matrix metalloprotease-1 receptor that promotes invasion and tumorigenesis of breast cancer cells [J].

Boire, A ;

Covic, L ;

Agarwal, A ;

Jacques, S ;

Sherifl, S ;

Kuliopulos, A .

CELL, 2005, 120 (03) :303-313

[7] Matrix metalloprotease inhibitors: design from structure [J].

Borkakoti, N .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2004, 32 :17-20

[8] Matrix metalloproteases: variations on a theme [J].

Borkakoti, N .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1998, 70 (01) :73-94

[9] The 1.8-Å crystal structure of a matrix metallaproteinase 8-barbiturate inhibitor complex reveals a previously unobserved mechanism for collagenase substrate recognition [J].

Brandstetter, H ;

Grams, F ;

Glitz, D ;

Lang, A ;

Huber, R ;

Bode, W ;

Krell, HW ;

Engh, RA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (20) :17405-17412

[10] Site-directed mutagenesis of the active site glutamate in human matrilysin: Investigation of its role in catalysis [J].

Cha, J ;

Auld, DS .

BIOCHEMISTRY, 1997, 36 (50) :16019-16024

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