Genetic and phylogenetic analysis of the non-structural proteins NS1, NS2 and NS3 of epizootic haemorrhagic disease virus (EHDV)

被引：38

作者：

Anthony, S. J. ^{[1
,2
]}

Maan, N. ^{[1
]}

Maan, S. ^{[1
]}

Sutton, G. ^{[2
]}

Attoui, H. ^{[1
]}

Mertens, P. P. C. ^{[1
]}

机构：

[1] Inst Anim Hlth, Vector Borne Dis Program, Surrey GU24 0NF, England

[2] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England

来源：

VIRUS RESEARCH | 2009年 / 145卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

EHDV; Non-structural protein; Virulence; Recombination; Viral evolution; MULTIPLE SEQUENCE ALIGNMENT; AFRICAN HORSESICKNESS VIRUS; BLUETONGUE VIRUS; INSECT CELLS; DEER; EXPRESSION; CLUSTAL; TUBULES; BACULOVIRUS; ASSIGNMENT;

D O I：

10.1016/j.virusres.2009.07.019

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three unique non-structural (NS) proteins are produced by Epizootic haemorrhagic disease virus (EHDV) during infection of a host cell; NS1, NS2 and NS3. This study presents a complete genetic and phylogenetic analysis of these proteins (and the genes that code for them) to allow comparison of the selective pressures acting on each. Accession numbers, gene and protein sizes, ORF positions, G + C contents, terminal hexanucleotides, start and stop codons and phylogenetic relationships are all presented. Unlike the core, or outer-coat proteins, there are no characteristic genetic or phylogenetic traits common to all of the EHDV NS proteins; indicating that each is evolving under different selection pressures. These differences are discussed. Evidence of genetic recombination in genome segment 8 (coding for NS2) is also presented, together with evidence of gene duplication and mutation, suggesting the EHDV genome may have evolved using mechanisms such as these. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：211 / 219

页数：9

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