Marked differences in differentiation propensity among human embryonic stem cell lines

被引：621

作者：

Osafune, Kenji ^{[1
,3
]}

Caron, Leslie ^{[1
,4
]}

Borowiak, Malgorzata ^{[1
,2
]}

Martinez, Rita J. ^{[1
,2
]}

Fitz-Gerald, Claire S. ^{[1
,2
]}

Sato, Yasunori ^{[5
]}

Cowan, Chad A. ^{[1
,4
,6
]}

Chien, Kenneth R. ^{[1
,4
,7
]}

Melton, Douglas A. ^{[1
,2
]}

机构：

[1] Harvard Univ, Stem Cell Inst, Cambridge, MA 02138 USA

[2] Harvard Univ, Howard Hughes Med Inst, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

[3] Japan Sci & Technol Agcy, ICORP Organ Regenerat Project, Meguro Ku, Tokyo 1538902, Japan

[4] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA

[5] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA

[6] Stowers Med Inst, Boston, MA 02114 USA

[7] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

来源：

NATURE BIOTECHNOLOGY | 2008年 / 26卷 / 03期

关键词：

D O I：

10.1038/nbt1383

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with > 100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.

引用

页码：313 / 315

页数：3

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