Superoxide anion handling by trout erythrocytes: A chemiluminescence study

被引：6

作者：

Gabbianelli, R

Santroni, AM

Concetti, A

Kantar, A

Falcioni, G

机构：

[1] UNIV CAMERINO,DEPT MOL CELLULAR & ANIM BIOL,I-62032 CAMERINO,MC,ITALY

[2] UNIV ANCONA,DEPT PEDIAT,I-60128 ANCONA,ITALY

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1996年 / 115卷 / 01期

关键词：

chemiluminescence; erythrocytes; density-separated fractions; hemolyzate; lucigenin; oxidative stress; plasma membrane; superoxide anion; trout;

D O I：

10.1016/S0742-8413(96)00118-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemiluminescence is being increasingly employed for evaluating oxidative stress in biological systems. Using this technique, we examined the oxidative stress in trout erythrocytes. Hemolysates and erythrocyte ghosts were studied in whole and in density-separated trout erythrocytes, after exposure to superoxide anion generated by the xanthine/xanthine oxidase system. The results show varying chemiluminescence response in the studied fractions, possibly reflecting different degrees of oxidation. Copyright (C) 1996 Elsevier Science Inc.

引用

页码：83 / 87

页数：5

共 13 条

[1] BOVELLI A, 1990, RED BLOOD CELL AGING, V307, P59
[2] SENESCENCE OF RED BLOOD-CELLS - PROGRESS AND PROBLEMS
CLARK, MR
[J]. PHYSIOLOGICAL REVIEWS, 1988, 68 (02) : 503 - 554
[3] SCAVENGER AND ANTIOXIDANT PROPERTIES OF 10 SYNTHETIC FLAVONES
COTELLE, N
BERNIER, JL
HENICHART, JP
CATTEAU, JP
GAYDOU, E
WALLET, JC
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 1992, 13 (03) : 211 - 219
[4] FALCIONI G, 1992, BIOCHEM INT, V28, P379
[5] GLUTATHIONE-PEROXIDASE AND OXIDATIVE HEMOLYSIS IN TROUT RED-BLOOD-CELLS
FALCIONI, G
CINCOLA, G
BRUNORI, M
[J]. FEBS LETTERS, 1987, 221 (02) : 355 - 358
[6] LUMINOL AND LUCIGENIN AS DETECTORS FOR O2.-
FAULKNER, K
FRIDOVICH, I
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 1993, 15 (04) : 447 - 451
[7] FIORINI R, 1990, BIOCHEM INT, V20, P715
[8] GRELLONI F, 1991, BIOCHEM INT, V25, P789
[9] KANTAR A, 1991, CURR THER RES, V54, P1
[10] LOWRY OH, 1951, J BIOL CHEM, V193, P265

← 1 2 →