Proteomics: quantitative and physical mapping of cellular proteins

被引：625

作者：

Blackstock, WP ^{[1
]}

Weir, MP ^{[1
]}

机构：

[1] Glaxo Wellcome Res & Dev Ltd, Biomol Struct Unit, Stevenage SG1 2NY, Herts, England

来源：

TRENDS IN BIOTECHNOLOGY | 1999年 / 17卷 / 03期

关键词：

D O I：

10.1016/S0167-7799(98)01245-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genome sequencing provides a wealth of information on predicted gene products (mostly proteins), but the majority of these have no known function. Two-dimensional gel electrophoresis and mass spectrometry have, coupled with searches in protein and EST databases, transformed the protein-identification process. The proteome is the expressed protein complement of a genome and proteomics is functional genomics at the protein level. Proteomics can be divided into expression proteomics, the study of global changes in protein expression, and cell-map proteomics, the systematic study of protein-protein interactions through the isolation of protein complexes.

引用

页码：121 / 127

页数：7

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