A new solid-phase synthesis of oligonucleotides 3′-conjugated with peptides

被引：31

作者：

De Napoli, L

Messere, A

Montesarchio, D

Piccialli, G

Benedetti, E

Bucci, E

Rossi, F

机构：

[1] Univ Naples Federico II, Dipartimento Chim Organ & Biol, I-80134 Naples, Italy

[2] Univ Naples Federico II, Dipartimento Chim, Ctr Studio Biocristallog, I-80134 Naples, Italy

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1999年 / 7卷 / 02期

关键词：

nucleic acids; peptides; solid-phase synthesis; HIV;

D O I：

10.1016/S0968-0896(98)00250-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A convenient 'on line' solid-phase synthesis of oligonucleotides conjugated at the 3'-end with peptides by means of a polymeric support linking the first nucleoside via the base has been developed. A 17-mer designed for antisense experiments against HIV-1, linking at the 3'-terminus the tripeptide Gly-Gly-His, was prepared in good yields and characterized by MALDI-TOF mass spectrometry. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：395 / 400

页数：6

共 17 条

[1] SYNTHESIS OF OLIGONUCLEOTIDE-PEPTIDE CONJUGATES CONTAINING A KDEL SIGNAL SEQUENCE [J].